Abstract
Herein we present methodological approaches for the identification and characterization of dopamine receptors in the subthalamic nucleus, a component nucleus of the basal ganglia, at pre-and postsynaptic locations and of their roles with an emphasis given to the dopamine D5 receptor subtype. This chapter focuses on the possible sources of divergence between electrophysiological studies and describes the pharmacological tools available for functional studies of this receptor. The procedures for single-cell reverse transcription PCR (polymerase chain reaction) identification of dopamine D5 receptor mRNA and the immunochemical detection of the receptor at cellular and subcellular levels are presented.
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Acknowledgments
We wish to thank the Regional Council of Aquitaine and Fondation de France who partly supported this work by grants 12006005 (FCAN scheme), and 2005013850 and 00016810, respectively. Financial support was also provided by CNRS and University of Bordeaux. D. Sibley (NINDS, Bethesda) and J. Drago (Monash University) allowed us to use the D5R and D1R mutant mice they engineered. J. Waddington (Royal College of Surgeons, Dublin) donated the D5R mutant mice we used to generate a colony. L.F received a PhD fellowship from MRT.
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Froux, L., Suarez-Boomgaard, D., Baufreton, J., Rivera, A., Garret, M., Taupignon, A. (2015). Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors. In: Tiberi, M. (eds) Dopamine Receptor Technologies. Neuromethods, vol 96. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2196-6_9
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DOI: https://doi.org/10.1007/978-1-4939-2196-6_9
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