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Chromosomal Mutagenesis pp 281–289Cite as

Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1239))

Abstract

The power of genome editing is increasingly recognized as it has become more accessible to a wide range of scientists and a wider range of uses has been reported. Nonetheless, an important practical aspect of the strategy is develop methods to increase the frequency of genome editing or methods that enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of cold-shock, exonucleases, surrogate markers, specialized donor vectors, and oligonucleotides to enhance the frequency of genome editing or to facilitate the identification of genome-edited cells.

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Authors and Affiliations

  1. Hematology/Oncology/Stem Cell Transplantation/Cancer Biology, Department of Pediatrics, School of Medicine, Stanford University, Lorry Lokey Stem Cell Research Building, G3045, MC 5462, 269 Campus Drive, Stanford, CA, 94305, USA

    Matthew Porteus M.D., Ph.D.

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  1. Matthew Porteus M.D., Ph.D.
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Correspondence to Matthew Porteus M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Genetics, Genome Engineering and iPSC Center, Washington University School of Medicine, St. Louis, Missouri, USA

    Shondra M. Pruett-Miller

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Porteus, M. (2015). Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells. In: Pruett-Miller, S. (eds) Chromosomal Mutagenesis. Methods in Molecular Biology, vol 1239. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1862-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1862-1_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1861-4

  • Online ISBN: 978-1-4939-1862-1

  • eBook Packages: Springer Protocols

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