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Agrobacterium Protocols

Volume 2

  • Book
  • © 2015

Overview

Editors:
  1. Kan Wang

    1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1224)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Industrial Plants

    1. Front Matter

      Pages 1-1
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    2. Brassica rapa

      • Tom Lawrenson, Cassandra Goldsack, Lars Ostergaard, Penny A. C. Hundleby née Sparrow
      Pages 3-10

    3. Cotton (Gossypium hirsutum L.)

      • Keerti S. Rathore, LeAnne M. Campbell, Shanna Sherwood, Eugenia Nunes
      Pages 11-23

    4. Jatropha (Jatropha curcas L.)

      • Devendra Kumar Maravi, Purabi Mazumdar, Shamsher Alam, Vaibhav V. Goud, Lingaraj Sahoo
      Pages 25-35

    5. Sesame (Sesamum indicum L.)

      • Sonia Kapoor, Sanjay S. Parmar, Manju Yadav, Darshna Chaudhary, Manish Sainger, Ranjana Jaiwal et al.
      Pages 37-45

    6. Sunflower (Helianthus annuus L.)

      • Laura M. Radonic, Dalia M. Lewi, Nilda E. López, H. Esteban Hopp, Alejandro S. Escandón, Marisa López Bilbao
      Pages 47-55

  3. Root Plants

    1. Front Matter

      Pages 57-57
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    2. Carrot (Daucus carota L.)

      • Owen S. D. Wally, Zamir K. Punja
      Pages 59-66

    3. Cassava (Manihot esculenta Crantz)

      • Simon E. Bull
      Pages 67-83

    4. Potato (Solanum tuberosum L.)

      • Venkateswari J. Chetty, Javier Narváez-Vásquez, Martha L. Orozco-Cárdenas
      Pages 85-96

    5. Taro (Colocasia esculenta (L.) Schott)

      • Xiaoling He, Susan C. Miyasaka, Maureen M. M. Fitch, Yun J. Zhu
      Pages 97-108

  4. Nuts and Fruits

    1. Front Matter

      Pages 109-109
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    2. Apricot (Prunus armeniaca L.)

      • César Petri, Nuria Alburquerque, Lorenzo Burgos
      Pages 111-119

    3. Blueberry (Vaccinium corymbosum L.)

      • Guo-Qing Song
      Pages 121-131

    4. Cherry

      • Guo-Qing Song
      Pages 133-142

    5. Chestnut, American (Castanea dentata (Marsh.) Borkh.)

      • Charles A. Maynard, Linda D. McGuigan, Allison D. Oakes, Bo Zhang, Andrew E. Newhouse, Lilibeth C. Northern et al.
      Pages 143-161

    6. Chestnut, European (Castanea sativa)

      • Elena Corredoira, Silvia Valladares, Ana M. Vieitez, Antonio Ballester
      Pages 163-176

    7. Grapevine (Vitis vinifera L.)

      • Laurent Torregrosa, Sandrine Vialet, Angélique Adivèze, Pat Iocco-Corena, Mark R. Thomas
      Pages 177-194

    8. Melon (Cucumis melo)

      • Satoko Nonaka, Hiroshi Ezura
      Pages 195-203
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Keywords

About this book

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.

Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.

Editors and Affiliations

  • Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, USA

    Kan Wang

Bibliographic Information

  • Book Title: Agrobacterium Protocols

  • Book Subtitle: Volume 2

  • Editors: Kan Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1658-0

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1657-3Published: 22 November 2014

  • Softcover ISBN: 978-1-4939-4544-3Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1658-0Published: 21 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVI, 378

  • Number of Illustrations: 6 b/w illustrations, 41 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Genetic Engineering

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