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Laser Ablation Sample Transfer for Mass Spectrometry Imaging

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1203))

Abstract

Infrared laser ablation sample transfer (IR-LAST) is a novel ambient sampling technique for mass spectrometry. In this technique, a pulsed mid-IR laser is used to ablate materials that are collected for mass spectrometry analysis; the material can be a solid sample or deposited on a sample target. After collection, the sample can be further separated or analyzed directly by mass spectrometry. For IR-LAST sample transfer tissue imaging using MALDI mass spectrometry, a tissue section is placed on a sample slide and material transferred to a target slide by scanning the tissue sample under a focused laser beam using transmission-mode (back side) IR laser ablation. After transfer, the target slide is analyzed using MALDI imaging. The spatial resolution is approximately 400 μm and limited by the spread of the laser desorption plume. IR-LAST for MALDI imaging provides several new capabilities including ambient sampling, area to spot concentration of ablated material, multiple ablation and analysis from a single section, and direct deposition on matrix-free nanostructured targets.

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References

  1. McDonnell LA, Heeren RMA (2007) Imaging mass spectrometry. Mass Spectrom Rev 26:606–643

    Article  PubMed  CAS  Google Scholar 

  2. Murayama C, Kimura Y, Setou M (2009) Imaging mass spectrometry: principle and application. Biophys Rev 1:131–139

    Article  CAS  Google Scholar 

  3. Boxer SG, Kraft ML, Weber PK (2009) Advances in imaging secondary ion mass spectrometry for biological samples. Annu Rev Biophys 38:53–74

    Article  PubMed  CAS  Google Scholar 

  4. Schwartz S, Caprioli R (2010) Imaging mass spectrometry: viewing the Future. In: Rubakhin SS, Sweedler JV (eds) Mass spectrometry imaging, vol. 656. Humana, New York, pp 3–19

    Chapter  Google Scholar 

  5. Schwamborn K, Caprioli RM (2010) MALDI imaging mass spectrometry—painting molecular pictures. Mol Oncol 4:529–538

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  6. Chughtai K, Heeren RMA (2010) Mass spectrometric imaging for biomedical tissue analysis. Chem Rev 110:3237–3277

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  7. Amstalden van Hove ER, Smith DF, Heeren RMA (2010) A concise review of mass spectrometry imaging. J Chromatogr 1217:3946–3954

    Article  CAS  Google Scholar 

  8. Chaurand P (2012) Imaging mass spectrometry of thin tissue sections: a decade of collective efforts. J Proteomics 75:4883–4892

    Article  PubMed  CAS  Google Scholar 

  9. Wu C, Dill AL, Eberlin LS et al (2013) Mass spectrometry imaging under ambient conditions. Mass Spectrom Rev 32:218–243

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  10. Takáts Z, Wiseman JM, Gologan B et al (2004) Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science 306:471–473

    Article  PubMed  Google Scholar 

  11. Dill AL, Ifa DR, Manicke NE et al (2009) Mass spectrometric imaging of lipids using desorption electrospray ionization. J Chromatogr B 877:2883–2889

    Article  CAS  Google Scholar 

  12. Esquenazi E, Dorrestein PC, Gerwick WH (2009) Probing marine natural product defenses with DESI-imaging mass spectrometry. Proc Natl Acad Sci U S A 106:7269–7270

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  13. Ifa DR, Wiseman JM, Song Q et al (2007) Development of capabilities for imaging mass spectrometry under ambient conditions with desorption electrospray ionization (DESI). Int J Mass Spectrom 259:8–15

    Article  CAS  Google Scholar 

  14. Kertesz V, Van Berkel GJ (2008) Improved imaging resolution in desorption electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 22:2639–2644

    Article  PubMed  CAS  Google Scholar 

  15. Kertesz V, Van Berkel GJ (2008) Scanning and surface alignment considerations in chemical imaging with desorption electrospray mass spectrometry. Anal Chem 80:1027–1032

    Article  PubMed  CAS  Google Scholar 

  16. Kertesz V, Van Berkel GJ, Vavrek M et al (2008) Comparison of drug distribution images from whole-body thin tissue sections obtained using desorption electrospray ionization tandem mass spectrometry and autoradiography. Anal Chem 80:5168–5177

    Article  PubMed  CAS  Google Scholar 

  17. Lane AL, Nyadong L, Galhena AS et al (2009) Desorption electrospray ionization mass spectrometry reveals surface-mediated antifungal chemical defense of a tropical seaweed. Proc Natl Acad Sci U S A 106:7314–7319

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  18. Wiseman JM, Ifa DR, Zhu Y et al (2008) Desorption electrospray ionization mass spectrometry: imaging drugs and metabolites in tissues. Proc Natl Acad Sci U S A 105:18120–18125

    Article  PubMed  PubMed Central  Google Scholar 

  19. Wu C, Ifa DR, Manicke NE et al (2009) Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization. Anal Chem 81:7618–7624

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  20. Manicke NE, Nefliu M, Wu C et al (2009) Imaging of lipids in atheroma by desorption electrospray ionization mass spectrometry. Anal Chem 81:8702–8707

    Article  PubMed  CAS  Google Scholar 

  21. Eberlin LS, Ifa DR, Wu C et al (2010) Three-dimensional visualization of mouse brain by lipid analysis using ambient ionization mass spectrometry. Angew Chem Int Ed 49:873–876

    Article  CAS  Google Scholar 

  22. Wiseman JM, Ifa DR, Song Q et al (2006) Tissue imaging at atmospheric pressure using desorption electrospray ionization (DESI) mass spectrometry. Angew Chem Int Ed 45:7188–7192

    Article  CAS  Google Scholar 

  23. Van Berkel GJ, Sanchez AD, Quirke JME (2002) Thin-layer chromatography and electrospray mass spectrometry coupled using a surface sampling probe. Anal Chem 74:6216–6223

    Article  PubMed  Google Scholar 

  24. Kertesz V, Ford MJ, Van Berkel GJ (2005) Automation of a surface sampling probe/electrospray mass spectrometry system. Anal Chem 77:7183–7189

    Article  PubMed  CAS  Google Scholar 

  25. Kertesz V, Van Berkel GJ (2010) Liquid microjunction surface sampling coupled with high-pressure liquid chromatography—electrospray ionization-mass spectrometry for analysis of drugs and metabolites in whole-body thin tissue sections. Anal Chem 82:5917–5921

    Article  PubMed  CAS  Google Scholar 

  26. Van Berkel GJ, Kertesz V, Koeplinger KA et al (2008) Liquid microjunction surface sampling probe electrospray mass spectrometry for detection of drugs and metabolites in thin tissue sections. J Mass Spectrom 43:500–508

    Article  PubMed  Google Scholar 

  27. Lanekoff I, Heath BS, Liyu A et al (2012) Automated platform for high-resolution tissue imaging using nanospray desorption electrospray ionization mass spectrometry. Anal Chem 84:8351–8356

    Article  PubMed  CAS  Google Scholar 

  28. Huang M-Z, Yuan C-H, Cheng S-C et al (2010) Ambient ionization mass spectrometry. Annu Rev Anal Chem 3:43–65

    Article  Google Scholar 

  29. Norris JL, Caprioli RM (2013) Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev 113:2309–2342

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  30. Guenther S, Römpp A, Kummer W et al (2011) AP-MALDI imaging of neuropeptides in mouse pituitary gland with 5 μm spatial resolution and high mass accuracy. Int J Mass Spectrom 305:228–237

    Article  CAS  Google Scholar 

  31. Peng IX, Ogorzalek Loo RR, Margalith E et al (2010) Electrospray-assisted laser desorption ionization mass spectrometry (ELDI-MS) with an infrared laser for characterizing peptides and proteins. Analyst 135:767–772

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  32. Nemes P, Barton AA, Li Y et al (2008) Ambient molecular imaging and depth profiling of live tissue by infrared laser ablation electrospray ionization mass spectrometry. Anal Chem 80:4575–4582

    Article  PubMed  CAS  Google Scholar 

  33. Shiea J, Huang M-Z, Hsu H-J et al (2005) Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids. Rapid Commun Mass Spectrom 19:3701–3704

    Article  PubMed  CAS  Google Scholar 

  34. Sampson J, Hawkridge A, Muddiman D (2006) Generation and detection of multiply-charged peptides and proteins by matrix-assisted laser desorption electrospray ionization (MALDESI) Fourier transform ion cyclotron resonance mass spectrometry. J Am Soc Mass Spectrom 17:1712–1716

    Article  PubMed  CAS  Google Scholar 

  35. Sampson J, Murray K, Muddiman D (2009) Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry. J Am Soc Mass Spectrom 20:667–673

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  36. Rezenom YH, Dong J, Murray KK (2008) Infrared laser-assisted desorption electrospray ionization mass spectrometry. Analyst 133:226–232

    Article  PubMed  CAS  Google Scholar 

  37. Nemes P, Vertes A (2007) Laser ablation electrospray ionization for atmospheric pressure, in vivo, and imaging mass spectrometry. Anal Chem 79:8098–8106

    Article  PubMed  CAS  Google Scholar 

  38. Murray KK (2006) Infrared MALDI. In: Caprioli RM, Gross ML (eds) Encyclopedia of mass spectrometry, vol 6. Elsevier, Amsterdam

    Google Scholar 

  39. Nemes P, Vertes A (2010) Atmospheric-pressure molecular imaging of biological tissues and biofilms by LAESI mass spectrometry. J Vis Exp 43:1–4

    Google Scholar 

  40. Robichaud G, Barry J, Garrard K et al (2013) Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) imaging source coupled to a FT-ICR mass spectrometer. J Am Soc Mass Spectrom 24:92–100

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  41. Shelley JT, Ray SJ, Hieftje GM (2008) Laser ablation coupled to a flowing atmospheric pressure afterglow for ambient mass spectral imaging. Anal Chem 80:8308–8313

    Article  PubMed  CAS  Google Scholar 

  42. Galhena AS, Harris GA, Nyadong L et al (2010) Small molecule ambient mass spectrometry imaging by infrared laser ablation metastable-induced chemical ionization. Anal Chem 82:2178–2181

    Article  PubMed  CAS  Google Scholar 

  43. Sabine Becker J (2013) Imaging of metals in biological tissue by laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS): state of the art and future developments. J Mass Spectrom 48:255–268

    Article  PubMed  CAS  Google Scholar 

  44. Kamali AS, Thompson JG, Bertman S et al (2011) Spray desorption collection of free fatty acids onto a solid phase microextraction fiber for trap grease analysis in biofuel production. Anal Method 3:683–687

    Article  CAS  Google Scholar 

  45. Venter AR, Kamali A, Jain S et al (2010) Surface sampling by spray-desorption followed by collection for chemical analysis. Anal Chem 82:1674–1679

    Article  PubMed  CAS  Google Scholar 

  46. Ovchinnikova OS, Kertesz V, Van Berkel GJ (2011) Combining laser ablation/liquid phase collection surface sampling and high-performance liquid chromatography−electrospray ionization-mass spectrometry. Anal Chem 83:1874–1878

    Article  PubMed  CAS  Google Scholar 

  47. Park S-G, Murray K (2011) Infrared laser ablation sample transfer for MALDI and electrospray. J Am Soc Mass Spectrom 22:1352–1362

    Article  PubMed  CAS  Google Scholar 

  48. Park S-G, Murray KK (2012) Infrared laser ablation sample transfer for on-line liquid chromatography electrospray ionization mass spectrometry. J Mass Spectrom 47:1322–1326

    Article  PubMed  CAS  Google Scholar 

  49. Huang M-Z, Jhang S-S, Cheng C-N et al (2010) Effects of matrix, electrospray solution, and laser light on the desorption and ionization mechanisms in electrospray-assisted laser desorption ionization mass spectrometry. Analyst 135:759–766

    Article  PubMed  CAS  Google Scholar 

  50. Park S-G, Murray KK (2013) Ambient laser ablation sampling for capillary electrophoresis mass spectrometry. Rapid Commun Mass Spectrom 27:1673–1680

    Article  PubMed  CAS  Google Scholar 

  51. Park S-G, Murray KK (2012) Infrared laser ablation sample transfer for MALDI imaging. Anal Chem 84:3240–3245

    Article  PubMed  CAS  Google Scholar 

  52. Fan X, Murray KK (2009) Wavelength and time-resolved imaging of material ejection in infrared matrix-assisted laser desorption. J Phys Chem A 114:1492–1497

    Article  Google Scholar 

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Acknowledgments

This work was supported by the National Science Foundation, Grant Number CHE-1152106.

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Authors and Affiliations

  1. Department of Chemistry, Louisiana State University, Baton Rouge, LA, 70803, USA

    Sung-Gun Park & Kermit K. Murray

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  1. Sung-Gun Park
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  2. Kermit K. Murray
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Corresponding author

Correspondence to Kermit K. Murray .

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Editors and Affiliations

  1. Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA

    Lin He

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Park, SG., Murray, K.K. (2015). Laser Ablation Sample Transfer for Mass Spectrometry Imaging. In: He, L. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 1203. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1357-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-1357-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1356-5

  • Online ISBN: 978-1-4939-1357-2

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