Abstract
Recent advances in small-angle X-ray scattering (SAXS) have led to the ability to model the glycans on glycoproteins and to obtain the low-resolution solution structures of complexes of lectins bound to multivalent glycan-presenting scaffolds. This progress in SAXS can respond to the increasing interest in the biological action of glycoproteins and lectins and in the design of multivalent glycan-based antagonists. Carbohydrates make up a significant part of the X-ray scattering content in SAXS and should be included in the model together with the protein, whose structure is most often based on a crystal structure or NMR ensemble, to give a far-improved fit with the experimental data. The modeling of the spatial positioning of glycans on proteins or in the architecture of lectin–glycan complexes delivers low-resolution structural information hitherto unmatched by any other method. SAXS data on the bacterial lectin FimH, strongly bound to heptyl α-d-mannose on a sevenfold derivatized β-cyclodextrin, permitted determination of the stoichiometry of the complex and the geometry of the lectin deposition on the multivalent β-cyclodextrin. The SAXS methods can be applied to larger complexes as the technique imposes no limit on the size of the macromolecular assembly in solution.
Key words
An erratum to this chapter is available at http://dx.doi.org/10.1007/978-1-4939-1292-6_47
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-1292-6_47
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Acknowledgements
This work was carried out with financial support to JB from the French Agence Nationale de la Recherche (ANR-12-BSV5-0016-01), the Centre National de la Recherche Scientifique, and the Minister̀e de l’Enseignement Supeŕieur et de la Recherche in France. SDW acknowledges the support of Marie-Curie Reintegration Grant. Measurements were made at beamlines X33 at the DORIS ring in Hamburg, Germany, and SWING at the French synchrotron SOLEIL, in Saint-Aubin.
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Weeks, S.D., Bouckaert, J. (2014). Small-Angle X-Ray Scattering to Obtain Models of Multivalent Lectin–Glycan Complexes. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_42
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DOI: https://doi.org/10.1007/978-1-4939-1292-6_42
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