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Hox Genes pp 319–348Cite as

Hox Protein Interactions: Screening and Network Building

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1196))

Abstract

Understanding the mode of action of Hox proteins requires the identification of molecular and cellular pathways they take part in. This includes to characterize the networks of protein-protein interactions involving Hox proteins. In this chapter we propose a strategy and methods to map Hox interaction networks, from yeast two-hybrid and high-throughput yeast two-hybrid interaction screening to bioinformatic analyses based on the software platform Cytoscape.

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Acknowledgements

We are grateful to the Belgian Foundation against Cancer and to the Télévie program coordinated by the Belgian National Fund for Scientific Research (FNRS) for their support. I.B. and B.L. held a FRIA fellowship from the FNRS. S.D. is a “Leon Fredericq” fellow. J.C.T. is Research Investigator of the FNRS.

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Authors and Affiliations

  1. Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5 box L7.07.10, Louvain-la-Neuve, 1348, Belgium

    Isabelle Bergiers, Barbara Lambert & René Rezsohazy

  2. Laboratory of Signaling and Protein Interactions, GIGA-R, University of Liege, Liège, 4000, Belgium

    Sarah Daakour & Jean-Claude Twizere

Authors
  1. Isabelle Bergiers
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  2. Barbara Lambert
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  3. Sarah Daakour
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  4. Jean-Claude Twizere
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  5. René Rezsohazy
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Corresponding author

Correspondence to René Rezsohazy .

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Editors and Affiliations

  1. IBDML, Marseille, France

    Yacine Graba

  2. Université Catholique de Louvain, Louvain-la-Neuve, Belgium

    René Rezsohazy

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Bergiers, I., Lambert, B., Daakour, S., Twizere, JC., Rezsohazy, R. (2014). Hox Protein Interactions: Screening and Network Building. In: Graba, Y., Rezsohazy, R. (eds) Hox Genes. Methods in Molecular Biology, vol 1196. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1242-1_20

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  • DOI: https://doi.org/10.1007/978-1-4939-1242-1_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1241-4

  • Online ISBN: 978-1-4939-1242-1

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