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Cancer Cell Signaling

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Martha Robles-Flores

    1. Universidad Nacional Autónoma de México, Mexico D.F., Mexico

    View editor publications

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  • Includes cutting-edge methods and protocols for studying cancer cell signaling
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1165)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Targeting Cancer Cell Metabolism as Anticancer Therapy

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Use of Biguanides to Improve Response to Chemotherapy

      • Vlad C. Sandulache, Liangpeng Yang, Heath D. Skinner
      Pages 3-9

    3. Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells via Metabolic Measurement and Clonogenic Survival

      • Sang Hyeok Woo, Vlad C. Sandulache, Liangpeng Yang, Heath D. Skinner
      Pages 11-18

    4. Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy

      • Nariman Ansari, Stefanie Hardung, Katharina Hötte, Stefanie Rakel, Patrick Antonietti, Donat Kögel et al.
      Pages 19-29

  3. Epigenetic Control of Cancer

    1. Front Matter

      Pages 31-31
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    2. Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer

      • Félix Recillas-Targa
      Pages 33-52

    3. Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF

      • Edgar González-Buendía, Rosario Pérez-Molina, Erandi Ayala-Ortega, Georgina Guerrero, Félix Recillas-Targa
      Pages 53-69

    4. Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers

      • Fermín Mar-Aguilar, Cristina Rodríguez-Padilla, Diana Reséndez-Pérez
      Pages 71-80

    5. Prostate Cancer Detection Using a Noninvasive Method for Quantifying miRNAs

      • Mauricio Rodríguez-Dorantes, A. Ivan Salido-Guadarrama, Pilar García-Tobilla
      Pages 81-87

    6. DNA Methylation Analysis of Steroid Hormone Receptor Genes

      • Ignacio Camacho-Arroyo, Valeria Hansberg-Pastor, Mauricio Rodríguez-Dorantes
      Pages 89-98

    7. Control of Oncogenic miRNA Function by Light-Activated miRNA Antagomirs

      • Colleen M. Connelly, Alexander Deiters
      Pages 99-114

    8. Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling

      • Yi Feng, Xiaowen Hu, Youyou Zhang, Dongmei Zhang, Chunsheng Li, Lin Zhang
      Pages 115-143

  4. Metastasis Promotion

    1. Front Matter

      Pages 145-145
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    2. Microvesicles as Mediators of Intercellular Communication in Cancer

      • Marc A. Antonyak, Richard A. Cerione
      Pages 147-173

    3. In Vivo Rat Model to Study Horizontal Tumor Progression

      • Catalina Trejo-Becerril, Enrique Pérez-Cárdenas, Alfonso Dueñas-González
      Pages 175-185

    4. An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice

      • César Alejandro Guzmán-Pérez, Alfredo Ibarra-Sánchez, José Luis Ventura-Gallegos, Claudia González-Espinosa, Jonathan García-Román, Alejandro Zentella-Dehesa
      Pages 187-195

    5. Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis

      • Qi Lv, Fang Hua, Zhuo-Wei Hu
      Pages 197-222

    6. Zebrafish-Based Systems Pharmacology of Cancer Metastasis

      • Yasuhito Shimada, Yuhei Nishimura, Toshio Tanaka
      Pages 223-238

  5. New Technologies in the Study of Cancer

    1. Front Matter

      Pages 239-239
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Keywords

About this book

Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment.

Editors and Affiliations

  • Universidad Nacional Autónoma de México, Mexico D.F., Mexico

    Martha Robles-Flores

Bibliographic Information

  • Book Title: Cancer Cell Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Martha Robles-Flores

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0856-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0855-4Published: 17 May 2014

  • Softcover ISBN: 978-1-4939-4648-8Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0856-1Published: 17 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 263

  • Number of Illustrations: 15 b/w illustrations, 27 illustrations in colour

  • Topics: Cancer Research

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