Abstract
This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Niesner RA, Hauser AE (2011) Recent advances in dynamic intravital multi-photon microscopy. Cytometry A 79A:789–798
Kuchmiy AA, Efimov GA, Nedospasov SA (2012) Methods for in vivo molecular imaging. Biochemistry (Mosc) 77:1339–1353
Giroir BP, Brown T, Beutler B (1992) Constitutive synthesis of tumor necrosis factor in the thymus. Proc Natl Acad Sci U S A 89:4864–4868
Harel Y, Silva M, Giroir B, Weinberg A, Cleary TB, Beutler B (1993) A reporter transgene indicates renal-specific induction of tumor necrosis factor (TNF) by shiga-like toxin. Possible involvement of TNF in hemolytic uremic syndrome. J Clin Invest 92:2110–2116
Bazzoni F, Kruys V, Shakhov A, Jongeneel CV, Beutler B (1994) Analysis of tumor necrosis factor promoter responses to ultraviolet light. J Clin Invest 93:56–62
Kravchenko VV, Kaufmann GF, Mathison JC, Scott DA, Katz AZ, Grauer DC et al (2008) Modulation of gene expression via disruption of NF-κB signaling by a bacterial small molecule. Science 321:259–263
Kuchmiy AA, Kruglov AA, Galimov AR et al (2011) New TNF reporter mouse to study TNF expression. Russ J Immunol 5:205–214
Siffrin V, Brandt AU, Radbruch H, Herz J, Boldakowa N, Leuenberger T et al (2009) Differential immune cell dynamics in the CNS cause CD4+ T cell compartmentalization. Brain 132:1247–1258
Herz J, Siffrin V, Hauser AE, Brandt AU, Leuenberger T, Radbruch H et al (2010) Expanding two-photon intravital microscopy to the infrared by means of optical parametric oscillator. Biophys J 98:715–723
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Shebzukhov, Y.V., Kuchmiy, A.A., Kruglov, A.A., Zipp, F., Siffrin, V., Nedospasov, S.A. (2014). Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label. In: Bayry, J. (eds) The TNF Superfamily. Methods in Molecular Biology, vol 1155. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0669-7_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0669-7_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0668-0
Online ISBN: 978-1-4939-0669-7
eBook Packages: Springer Protocols