Abstract
Coronaviruses are ubiquitous in nature and cause several diseases in infected hosts (Robb and Bond, 1979a). The genomic RNA of coronaviruses has been extensively studied in recent years (Lomneczi and Kennedy, 1977; Yogo et al., 1977; Lai and Stohlman, 1978; Wege et al., 1978; Macnaughton and Madge, 1978; Guy and Brian, 1979). These studies have shown that the coronavirus genome is a large, single stranded RNA which is polyadenylated and of positive polarity.
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Keywords
- Infectious Bronchitis Virus
- Nucleocapsid Protein
- Mouse Hepatitis Virus
- Subgenomic RNAs
- Tris Acetate Buffer
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Bailey, J.M., and Davidson, N., 1976, Methylinercury as a reversible denaturing agent for agarose gel electrophoresis, Anal. Biochem., 70: 75.
Bond, C.W., Leibowitz, J. L, and Robb, J.A., 1979, Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V, Virology, 94: 371.
Borun, T.W., Scharff, M.D., and Robbin, E., 1967, Preparation of mammalian polyribosomes with the detergent Nonidat P-40, Biochim. Biophys. Acta, 149: 302.
Cleveland, D.W., Fischer, S.G., Kirschner, M.W., and Laemmli, U.K., 1977, Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis, J. Biol. Chem., 252: 1102.
Gibson, W., 1974, Polyoma virion proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis, Virology, 62: 319.
Guy, J.S., and Brian, D.A., 1979, Bovine coronavirus genome, J. Virol., 29: 293.
Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227: 680.
Lai, M.M.C., and Stohlman, S.A., 1978, RNA of mouse hepatitis virus, J. Virol., 26: 236.
Laskey, R.A., and Mills, A.D., 1977, Enhanced autoradiographic detection of 32p and 125I using intensifying screens and hypersensitized film. FEBS Letters, 82: 314.
Lee, Y.F., Kitamura, N., Nomoto, A., and Wimmer, E., 1979, Sequence studies of poliovirus type 1, type 2, and two type 1 defective interfering particles RNAs1 and fingerprint of the poliovirus type 3 genome, J. Gen. Virol., 44: 311.
Lomniczi, B., and Kennedy, I., 1977, Genome of infectious bronchitis virus, J. Virol., 24: 99.
Macnaughton, M.R., and Madge, M.H., 1978, The genome of human coronavirus strain 229E, J. Gen. Virol., 39: 497.
McMaster, G.K., and Carmichael, G.C., 1977, Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange, Proc. Natl. Acad. Sci. U.S.A., 75: 4835.
Pelham, H.R.B., and Jackson, R.J., 1976, An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem., 67: 247.
Robb, J.A., and Bond, C.W., 1979a, Coronaviridae, in: “Comprehensive Virology,” vol. 14, H. Fraenkel-Conrat and R.R. Wagner, eds., Plenum Publishing Corp., New York.
Robb, J.A., and Bond, C.W., 1979b, Pathogenic murine coronaviruses. I. Characterization of biological behavior in vitro and virus specific intracellular RNA of strongly neutotropic JHMV and weakly neurotropic A59V viruses, Virology, 94: 352.
Siddell, S.G., Wege, H., Barthel, A., and terMeulen, V., 1980, Coronavirus JHM:Cell-free synthesis of structural protein p 60, J. Virol., 33: 10.
Stern, D.F., and. Kennedy, S.I.T., 1980, Coronavirus multiplication strategy. I. Identification and characterization of virusspecified RNA, J. Virol., 34: 665.
Stohlman, S.A., and Lai, M.M.C., 1979, Phosphoproteins of murine hepatitis viruses, Virology, 32: 672.
Sturman, L.S., and Takemoto, K.K., 1972, Enhanced growth of a murine coronavirus in transformed mouse cells. Infection & Immunity, 6: 501.
Wege, H., Muller, A., and terMeulen, V., 1978, Genomic RNA of the murine coronavirus JHM, J. Gen. Virol., 41: 217.
Wilt, F.H., 1977, The dynamics of maternal poly(A)-containing mRNA in fertilized sea urchin eggs, Cell, 11: 673.
Yogo, Y., Hirano, N., Hino, S., Shibuta, H., and Matumoto, M., 1977, Polyadenylate in the virion RNA of mouse hepatitis virus, J. Biochem., 82: 1103.
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Leibowitz, J.L., Weiss, S.R. (1981). Murine Coronavirus RNA. In: ter Meulen, V., Siddell, S., Wege, H. (eds) Biochemistry and Biology of Coronaviruses. Advances in Experimental Medicine and Biology, vol 142. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0456-3_19
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DOI: https://doi.org/10.1007/978-1-4757-0456-3_19
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