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Purification of Myocardial Adenosine Kinase Using Affinity and Ion-Exchange Chromatography

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Purine Metabolism in Man—III

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 122B))

Abstract

Myocardial adenosine kinase (AK; EC 2.7.1.20) presumably plays a key role in the maintenance of adequate adenine nucleotide levels in the heart cell1–3. In order to study this enzyme in detail, we purified rat-heart AK to apparent homogeneity after a previous report on partial purification from this source4. The method presented here includes elution of AK from a 5′-AMP-Sepharose 4B column with a buffer containing adenosine. The endogenous adenosine in the fractions altered the specific activity of the radioactive substrate in the AK assay. This could be corrected for by means of HPLC adenosine measurements.

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References

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Authors and Affiliations

  1. Cardiochemical Laboratory, Thoraxcenter, Erasmus University Rotterdam, Rotterdam, The Netherlands

    Martin P. Uitendaal, Jan W. De Jong, Eef Harmsen & Elisabeth Keijzer

Authors
  1. Martin P. Uitendaal
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  2. Jan W. De Jong
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  3. Eef Harmsen
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  4. Elisabeth Keijzer
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Editor information

Editors and Affiliations

  1. Fundación Jiménez Díaz, Madrid, Spain

    Aurelio Rapado

  2. M.R.C. Clinical Research Centre, Harrow, England

    R. W. E. Watts

  3. Department of Human Genetics, University of Nijmegen Faculty of Medicine, Nijmegen, The Netherlands

    Chris H. M. M. De Bruyn

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© 1980 Plenum Press, New York

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Uitendaal, M.P., De Jong, J.W., Harmsen, E., Keijzer, E. (1980). Purification of Myocardial Adenosine Kinase Using Affinity and Ion-Exchange Chromatography. In: Rapado, A., Watts, R.W.E., De Bruyn, C.H.M.M. (eds) Purine Metabolism in Man—III. Advances in Experimental Medicine and Biology, vol 122B. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8559-2_65

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  • DOI: https://doi.org/10.1007/978-1-4684-8559-2_65

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-8561-5

  • Online ISBN: 978-1-4684-8559-2

  • eBook Packages: Springer Book Archive

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