Abstract
A question of major interest in considering myoblast therapy is whether the gene product dystrophin, provided by the introduced myoblasts, can contribute to the function of the myofiber into which the cells fuse. As shown in several papers in this volume, there is now ample evidence that injected myoblasts can fuse with myofibers and produce dystrophin, but the question of whether that dystrophin can restore muscle function still remains. In this regard, a major consideration is whether dystrophin can gain access to distant sites within the myofiber or remains localized in the vicinity of the nucleus that encoded it.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ralston, E. and Z.W. Hall, Transfer of a protein encoded by a single nucleus to nearby nuclei in multinucleated myotubes, Science, 244: 1066 (1989).
Blau, H.M., C.-P. Chiu and C. Webster, Cytoplasmic activation of human nuclear genes in stable heterocaryons, Cell 32: 117 (1983).
Blau, H.M., G.K. Pavlath, E.C. Hardeman, C.-P. Chiu, L. Silberstein, S.G. Webster, S.C. Miller and C. Webster, Plasticity of the differentiated state, Science 230: 758 (1985).
Walsh, F.S., G. Dickson, S.E. Moore, C.H. Barton, Unmasking N-CAM, Nature 339: 516 (1989).
Pavlath, G.K., K. Rich, S.G. Webster and H.M. Blau, Localization of muscle gene products in nuclear domains, Nature 337: 570 (1989).
Watkins, J.F. and D.M. Grace, Studies on the surface antigens of interspecific mammalian cell heterokaryons, J. Cell Sci. 2: 193 (1967).
Harris, H., E. Sidebottom, D.M. Grace and M.E. Bramwell, The expression of genetic information: A study with hybrid animal cells, J. Cell Sci. 4: 499 (1969).
Frye, L.D. and M. Edidin, The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons, J. Cell Sci., 7: 319 (1970).
Tassin, A.-M., B. Mara and M. Bornens, Fate of microtubule - organizing centers during myogenesis in vitro,J. Cell Biol. 100: 35 (1985).
Partridge, T.A., M. Grounds and J.C. Sloper, Evidence of fusion between host and donor myoblasts in skeletal muscle grafts, Nature 273: 306 (1978).
Gearhart, J.D. and B. Mintz, Clonal origins of somites and their muscle derivatives; evidence from allophenic mice,Develop. Biol. 29: 27 (1972).
Hausmanowa- Petrusewicz, I., I. Niebroj- Dobosz, J. Borkowska, E. Lukasik and D. Liszewska-Pfejfer, Carrier detection in Duchenne dystrophy in: “Pathogenesis of Human Muscular Dystrophies,” L.P. Rowland, ed., Excerpta Medica, Oxford (1977).
Koenig, M., A.P. Monaco and L.M. Kunkel, The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein, Cell 53: 219 (1988).
Miller, S.C., G.K. Pavlath, B.T. Blakely and H.M. Blau, Muscle cell components dictate hepatocyte gene expression and the distribution of the Golgi apparatus in heterokaryons, Genes Devel., 2: 330 (1988).
Silberstein, L., S.G. Webster, M. Travis and H.M. Blau, Developmental progression of myosin gene expression in cultured muscle cells, Cell, 46: 1075 (1986).
Webster, C., L. Silberstein, A.P. Hays and H.M. Blau, Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy, Cell, 52: 502 (1988).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this chapter
Cite this chapter
Blau, H.M., Pavlath, G.K., Rich, K., Webster, S.G. (1990). Localization of Muscle Gene Products in Nuclear Domains: Does this Constitute a Problem for Myoblast Therapy?. In: Griggs, R.C., Karpati, G. (eds) Myoblast Transfer Therapy. Advances in Experimental Medicine and Biology, vol 280. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5865-7_19
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5865-7_19
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5867-1
Online ISBN: 978-1-4684-5865-7
eBook Packages: Springer Book Archive