Abstract
Previous studies of the membrane fusion process have permitted the characterization of membrane permeability changes concomitant with MHV-induced cytopathology. One indication of membrane permeability in MHV-infected cells is their sensitivity to translational inhibition by the normally impermeable amino-glycoside, hygromycin B (Macintyre, G., Wong, F. and Anderson, R. (1989) J. Gen. Virol. 70, 763–768). In the present study, we examine the hygromycin B sensitivity of acutely infected mouse fibroblast L-2 cell and macrophage cultures as well as persistently infected mouse fibroblast LM-K cell cultures. The results suggest that membrane permeability alterations (as indicated by hygromycin B sensitivity) are a common feature of these MHV infections. Hygromycin B “cured” persistently infected LM-K cells as indicated by the absence of detectable virus antigen by immunofluorescence and by the absence of infectious virus even after removal of the drug or co-cultivation with untreated L-2 cells. The results argue against the maintenance of MHV infection by a mechanism involving latently or non-cytolytically infected cells. We conclude therefore that at least one mechanism for MHV persistence depends on virus propagation by cytolytic infection of a small, dynamically changing, fraction of the total cells present in culture.
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Stohlman, S.A., Sakaguchi, A.Y. and Weiner, LP. (1979) Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion. Virology 98:448–455.
Holmes, K.V. and Behnke, J.N. (1981) Evolution of a Coronavirus during persistent infection in vitro. Adv. Exp. Med. Biol. 142:287–299.
Jackson, D., Percy, D. and Morris, V.L. (1984) Characterization of murine hepatitis virus (JHM) RNA from rats with experimental encephalomyelitis. Virology 137:297–304.
Taguchi, F., Siddell, S., Wege, H., Massa, P. and ter Meulen, V. (1987) Characterization of JHMV variants isolated from rat brain and cultured neural cells after wild type JHMV infection. Adv. Exp. Med. Biol. 218:343–349.
Lucas, A., Flintoff, W., Anderson, R., Percy, D., Coulter, M. and Dales, S. (1977) In vivo and in vitro models of demyelinating diseases: Tropism of the JHM strain of murine hepatitis virus for cells of glial origin. Cell 12:553–560.
Lucas, A., Coulter, M., Anderson, R., Dales, S. and Flintoff, W. (1978) In vivo and in vitro models of demyelinating diseases. II. Persistence and host-regulated thermosensitivity in cells of neural derivation infected with mouse hepatitis and measles viruses. Virology 88:325–337.
Mizzen, L., Cheley, S., Rao, M., Wolf, R. and Anderson, R. (1983) Fusion resistance and decreased infectability as major host cell determinants of Coronavirus persistence. Virology 128:407–417.
Bailey, O.T., Pappenheimer, A.M. and Cheever, F.S. (1949) A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. J. Exptl. Med. 90:195–212.
Weiner, L.P., Johnson, R.T. and Herndon, R.M. (1973) Viral infections and demyelinating diseases. New Engl. J. Med. 288:1103–1110.
Haspel, M.V., Lampert, P.W. and Oldstone, M.B.A. (1978) Temperature-sensitive mutants of mouse hepatitis virus produce a high incidence of demyelination. Proc. Natl. Acad. Sci. U.S.A. 75:4033–4036.
Nagashima, K., Wege, H., Meyermann, R. and ter Meulen, V. (1978) Demyelinating encephalomyelitis induced by long-term Coronavirus infection in rats. A preliminary report. Acta Neuropathol. 45:205–213.
Sorensen, O., Percy, D. and Dales, S. (1980) In vivo and in vitro models of demyelinating diseases. III. JHM virus infection of rats. Arch. Neurol. 37:478–484.
Sorensen, O., Beushausen, S., Puchalski, S., Cheley, S., Anderson, R., Coulter-Mackie, M. and Dales, S. (1984) In vitro and in vivo models of demyelinating diseases -VIII: genetic, immunologic and cellular influences on JHM virus infection of rats. Adv. Exp. Med. Biol. 173:279–298.
Mizzen, L., Macintyre, G., Wong, F. and Anderson, R. (1987) Translational regulation in mouse hepatitis virus infection is not mediated by altered intracellular ion concentrations. J. Gen. Virol. 68:2143–2151.
Macintyre, G., Wong, F. and Anderson, R. (1989) A model for persistent murine Coronavirus infection involving maintenance via cytopathically infected cell centres. J. Gen. Virol. 70:763–768.
Rothfels, K.H., Axelrad, A.A., Siminovitch, L, McCulloch, E.A. and Parker, R.C. (1959) The origin of altered cell lines from mouse, monkey and man as indicated by chromosome and transplantation studies. Can. Cancer Conf. 3:189–214.
Kit, S., Dubbs, D.R., Piekarski, L.J. and Hsu, T.C. (1963) Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31:297–312.
Manaker, R.A., Piczak, C.V., Miller, A.A. and Stanton, M.F. (1961) A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27:29–44.
Taguchi, F., Yamaguchi, R., Makino, S. and Fujiwara, K. (1981) Correlation between growth potential of mouse hepatitis viruses in macrophages and their virulence for mice. Infect. Immun. 34:1059–1061.
Benedetto, A., Rossi, G.B., Amici, C., Belardelli, F., Cioe, L, Carruba, G. and Carrasco, L. (1980) Inhibition of animal virus production by means of translation inhibitors unable to penetrate normal cells. Virology 106:123–132.
Cameron, J.M., Clemens, M.J., Gray, M.A., Menzies, D.E., Mills, B.J., Warren, A.P. and Pasternak, C.A. (1986) Increased sensitivity of virus-infected cells to inhibitors of protein synthesis does not correlate with changes in plasma membrane permeability. Virology 155:534–544.
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© 1990 Plenum Press, New York
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Macintyre, G., Kooi, C., Wong, F., Anderson, R. (1990). On the Membrane Cytopathology of Mouse Hepatitis Virus Infection as Probed by a Semi-Permeable Translation-Inhibiting Drug. In: Cavanagh, D., Brown, T.D.K. (eds) Coronaviruses and their Diseases. Advances in Experimental Medicine and Biology, vol 276. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5823-7_10
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DOI: https://doi.org/10.1007/978-1-4684-5823-7_10
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