Abstract
A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.
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Keywords
- Restriction Enzyme Recognition Site
- Quantitative Amount
- Specific Restriction Enzyme
- Intermediate Reaction Product
- Marker Mutation
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Hartvig, L., and Christiansen, J., 1996, Intrinsic termination of T7 RNA polymerase mediated either by RNA or DNA, EMBO J. 15:4767–4774.
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© 1998 Springer Science+Business Media New York
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Herold, J., Thiel, V., Siddell, S.G. (1998). A Strategy for the Generation of Infectious RNAs and Autonomously Replicating RNAs Based on the HCV 229E Genome. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_34
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_34
Publisher Name: Springer, Boston, MA
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