Abstract
Follicular dendritic cells (FDCs) are immune accessory cells, located in lymphoid follicles1. FDCs can trap and retain antigen antibody complexes on their long slender dendrites for a long periods of time2 and it appears that antigen retained on FDCs can induce primed B cells to make specific antibody3. FDCs can form liposome-like immune complexes coating bodies (iccosomes)4. These iccosomes may be released and then engulfed by adjacent B lymphocytes. The engulfed iccosomes appeared to disintegrate in the B cells close to Golgi complex. We believed the iccosomal Ag is being processed and Ag fragments are being delivered to the B cell surface for presentation to T cells5. Antibody forming cells (AFC) emerge in germinal centers 3 to 4 days after antigen challenge6. The coincidence of iccosome release and AFC emergence led to the hypothesis that antigen bearing iccosomes might be able to induce B cells to differentiate into AFC7. In this study, we sought to test the hypothesis that iccosomal Ag is involved in the induction of specific Ab production. Iccosomes were prepared from FDCs 3 days after OVA challenge by sonicating FDCs or shaking them in the upper chamber of a transwell apparatus with a 3.0 um pore diameter filter and collecting icccosomes from the lower chamber of the apparatus. Both iccosome preparations were able to induce OVA primed lymphocytes to produce anti-OVA. However intact FDCs were critical for optimal Ab production.
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Wu, J., Qin, D., Burton, G.F., Szakal, A.K., Tew, J.G. (1995). Iccosomes and Induction of Specific Antibody Production in Vitro. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_69
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DOI: https://doi.org/10.1007/978-1-4615-1971-3_69
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