Abstract
Aldose reductase (aditol NADP+ oxidoreductase, EC 1.1.1.21) with sorbitol dehydrogenase (1-iditol dehydrogenase, EC 1.1.1.4) together form the polyol pathway where glucose is converted to fructose through the sugar alcohol sorbitol. In diabetes the increased flux of glucose through the polyol pathway results in the accumulation of sorbitol which is linked to the onset of various diabetic complications such as cataract formation, retinopathy, neuropathy and nephropathy (Kinoshita, 1974; Kador, 1988; Kinoshita et al., 1990; Kador et al., 1990). This has spurred the world-wide interest in the development of aldose reductase inhibitors as a new approach toward the treatment of diabetic complications.
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The material in this chapter was not presented at the meeting, but is based upon an extension of work presented at the 1990 workshop (Carper et al., 1990).
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Sato, S., Old, S., Carper, D., Kador, P.F. (1995). Purification and Characterization of Recombinant Human Placental and Rat Lens Aldose Reductases Expressed in Escherichia Coli. In: Weiner, H., Holmes, R.S., Wermuth, B. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 5. Advances in Experimental Medicine and Biology, vol 372. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1965-2_32
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