Abstract
The mouse hepatitis virus (MHV) genome is a 32kb message sense single stranded RNA. Studies with defective interfering RNAs have shown that the 3′ terminal 447nt are required for replication (Kim et al., 1993; Lin et al., 1993). Previous studies have identified two host protein binding elements within the 3’ terminal 166nt of the 3′UTR. The first of these is located at nucleotides 154-129 [assigning position 1 to the first nucleotide 5′ of the poly (A)] tail and the second is located within the 3′ terminal 42 nucleotides (Liu et al, 1997; Yu and Leibowitz, 1995). Both elements contain the conserved motif UGAARNGAAGUU which is required for host protein binding and for DI RNA replication (Yu and Leibowitz, 1995; Liu et al., 1997). In the present study we further identify nucleotides involved in host protein binding and explore the role of RNA secondary structure in this process.
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Keywords
- Secondary Structure
- Mouse Hepatitis Virus
- Secondary Structure Model
- Protein Binding Activity
- Wild Type Structure
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© 2001 Springer Science+Business Media New York
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Johnson, R.F., Leibowitz, J.L. (2001). Mutagenesis of the 3′42 Nucleotide Host Protein Binding Element of the MHV 3′UTR. In: Lavi, E., Weiss, S.R., Hingley, S.T. (eds) The Nidoviruses. Advances in Experimental Medicine and Biology, vol 494. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1325-4_72
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DOI: https://doi.org/10.1007/978-1-4615-1325-4_72
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