Abstract
Small animals are widely used for the identification of new therapeutic targets and the evaluation of potential anticancer therapies. To study tumors and metastasis in longitudinal studies of tumor progression, fast noninvasive and easy-to-handle imaging modalities are required. Here, techniques for the analysis of tumors and metastases by ultrasound imaging are described and the potential technical pitfalls are discussed.
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References
Ariff B, Lloyd CR, Khan S, Shariff M, Thillainayagam AV, Bansi DS, Khan SA, Taylor-Robinson SD, Lim AK (2009) Imaging of liver cancer. World J Gastroenterol 15:1289–1300
Blomley MJ, Eckersley RJ (2002) Functional ultrasound methods in oncological imaging. Eur J Cancer 38:2108–2115
Rizzatto G, Chersevani R, Abbona M, Lombardo VL, Macorig D (1997) High-resolution sonography of breast carcinoma. Eur J Radiol 24:11–19
Voit C, Schoengen A, Schwurzer M, Weber L, Mayer T, Proebstle TM (1999) Detection of regional melanoma metastases by ultrasound B-scan, cytology or tyrosinase RT-PCR of fine-needle aspirates. Br J Cancer 80:1672–1677
Phoon CK, Turnbull DH (2003) Ultrasound biomicroscopy-Doppler in mouse cardiovascular development. Physiol Genomics 14:3–15
Srinivasan S, Baldwin HS, Aristizabal O, Kwee L, Labow M, Artman M, Turnbull DH (1998) Noninvasive, in utero imaging of mouse embryonic heart development with 40-MHz echocardiography. Circulation 98:912–918
Wirtzfeld LA, Wu G, Bygrave M, Yamasaki Y, Sakai H, Moussa M, Izawa JI, Downey DB, Greenberg NM, Fenster A, Xuan JW, Lacefield JC (2005) A new three-dimensional ultrasound microimaging technology for preclinical studies using a transgenic prostate cancer mouse model. Cancer Res 65:6337–6345
Gee MS, Saunders HM, Lee JC, Sanzo JF, Jenkins WT, Evans SM, Trinchieri G, Sehgal CM, Feldman MD, Lee WM (2001) Doppler ultrasound imaging detects changes in tumour perfusion during antivascular therapy associated with vascular anatomic alterations. Cancer Res 61:2974–2982
Xie Q, Thompson R, Hardy K, DeCamp L, Berghuis B, Sigler R, Knudsen B, Cottingham S, Zhao P, Dykema K, Cao B, Resau J, Hay R, Vande Woude GF (2008) A highly invasive human glioblastoma pre-clinical model for testing therapeutics. J Transl Med 6:77
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Didié, M., Zimmermann, WH. (2014). Ultrasound Techniques for the Detection of Tumors and Metastases in Small Animals. In: Dwek, M., Schumacher, U., Brooks, S. (eds) Metastasis Research Protocols. Methods in Molecular Biology, vol 1070. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-8244-4_13
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DOI: https://doi.org/10.1007/978-1-4614-8244-4_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4614-8243-7
Online ISBN: 978-1-4614-8244-4
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