Abstract
Quantum dots (QDs) are the semiconductor crystal with a nanometer particle size that emit fluorescence of a size-dependent wavelength. In this study, we examined whether l-cysteine-capped CdTe quantum dots (QD580, diameter ~4 nm) might be used as an optical probe for intracellular oxygen (O2) in cultured cells. QD580 was successfully introduced in cultured COS-7 cells by incubating cells with 10 nM QD580 for 5–60 min at 37°C. Cells were exposed to 20 % O2 (0.5 h), then 0.5 % O2 or 20 % O2 (1 h), and finally 20 % O2 (0.5 h) gases. We found significant increases in the fluorescence intensity at 0.5 % O2. However, when compared with QD580 in buffer solution, QD580 fluorescence in cells was considerably weak and vulnerable to repeated excitation light exposures. The present study demonstrated the potential of l-cysteine-capped CdTe QDs as a nanoscale probe for intracellular O2 in cultured cells. Further improvement of the QD is necessary for quantitative assessment of O2 in the cell.
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References
Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281(5385):2016–2018
Xia Y, Zhang T, Diao X, Zhu C (2007) Measurable emission color change: size-dependent reversible fluorescence quenching of CdTe quantum dots by molecular oxygen. Chem Lett 36(2):242–243
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This work was supported by KAKENHI 23500520 to ET.
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© 2013 Springer Science+Business Media New York
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Higashi, K., Jin, T., Takahashi, E. (2013). Oxygen-Sensitive Quantum Dots for Possible Nanoscale Oxygen Imaging in Cultured Cells. In: Van Huffel, S., Naulaers, G., Caicedo, A., Bruley, D.F., Harrison, D.K. (eds) Oxygen Transport to Tissue XXXV. Advances in Experimental Medicine and Biology, vol 789. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7411-1_50
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DOI: https://doi.org/10.1007/978-1-4614-7411-1_50
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