Abstract
Methods are described for the transformation of sugar and loblolly pine cells by Agrobacterium. DNA transfer and expression is established by DNA-DNA and DNA-RNA hybridization data, opine identification, kanamycin resistance and neomycin phosphotransferase activity, growth characteristics of gall formation, and hormone autotrophy. Some of the difficulties inherent in conifer transformation systems are discussed, including strain selection, callus proliferation from galls, NPTII assays, and Southern hybridizations. A general strategy for the development of Agrobacterium transformation systems for woody plants is presented.
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© 1988 Plenum Press, New York
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Stomp, AM. et al. (1988). Development of a DNA Transfer System for Pines. In: Hanover, J.W., Keathley, D.E., Wilson, C.M., Kuny, G. (eds) Genetic Manipulation of Woody Plants. Basic Life Sciences, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1661-9_14
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DOI: https://doi.org/10.1007/978-1-4613-1661-9_14
Publisher Name: Springer, Boston, MA
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