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Cellular Therapies in Vascularized Composite Allograft

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Plastic and Reconstructive Surgery

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Abstract

The preclinical experimental models of donor bone marrow transplantation (BMT) for chimerism induction have rapidly developed in the past years. The presence of mixed chimerism in solid organs and in vascularized composite allograft (VCA) transplantations is on focus as tolerance-inducing strategy for allograft survival with minimal or without long-lasting immunosuppression allowing for widespread application in the clinic. In this Chapter we summarize our experience of cellular therapies with donor hematopoietic cells for chimerism induction in VCA. We introduce different alternative methods of bone marrow cell (BMC) delivery including: (1) standard intravenous, (2) intraosseous, and into immunoprivileged compartments (3) under kidney capsule, (4) into testis, and (5) intrathecal infusion, for chimerism induction. The efficacy of donor BMT for chimerism induction was tested on limb, vascularised skin and face transplant experimental models.

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Abbreviations

BMC:

Bone marrow cells

BMT:

Bone marrow transplantation

CsA:

Cyclosporine A

GvHD:

Graft-versus-host disease

HSCT:

Hematopoietic stem cells transplantation

LBN:

Lewis-Brown Norway

mAb:

Monoclonal antibody

MACS:

Magnetic assay cell sorting

MHC:

Major histocompatibility complex

VCA:

Vascularized composite allografts

αβ-TCR:

αβ- T-cell receptor

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Author information

Authors and Affiliations

  1. Department of Clinical Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wroclaw, Poland

    Aleksandra Klimczak PhD, DSc

  2. Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH, USA

    Arkadiusz Jundzill MD

Authors
  1. Aleksandra Klimczak PhD, DSc
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  2. Arkadiusz Jundzill MD
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Corresponding author

Correspondence to Aleksandra Klimczak PhD, DSc .

Editor information

Editors and Affiliations

  1. Department of Orthopaedics, University of Illinois at Chicago, Chicago, Illinois, USA

    Maria Z. Siemionow

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Klimczak, A., Jundzill, A. (2015). Cellular Therapies in Vascularized Composite Allograft. In: Siemionow, M. (eds) Plastic and Reconstructive Surgery. Springer, London. https://doi.org/10.1007/978-1-4471-6335-0_74

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  • DOI: https://doi.org/10.1007/978-1-4471-6335-0_74

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  • Print ISBN: 978-1-4471-6334-3

  • Online ISBN: 978-1-4471-6335-0

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