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miRNAs Need a Trim

Regulation of miRNA Activity by Trim-NHL Proteins

  • Chapter
Regulation of microRNAs

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 700))

Abstract

Trim-NHL proteins are defined by RING, B-Box and Coiled-coil protein motifs (referred to collectively as the Trim domain) coupled to an NHL domain. The C. elegans, D. melanogaster, mouse and human Trim-NHL proteins are potential and in several cases confirmed, E3 ubiquitin ligases. Current research is focused on identifying targets and pathways for Trim-NHL-mediated ubiquitination and in assessing the contribution of the NHL protein-protein interaction domain for function and specificity. Several Trim-NHL proteins were discovered in screens for developmental genes in model organisms; mutations in one of the family members, Trim32, cause developmental disturbances in humans. In most instances, mutations that alter protein function map to the NHL domain. The NHL domain is a scaffold for the assembly of a translational repressor complex by the Brat proto-oncogene, a well-studied family member in Drosophila. The link to translational control is common to at least four Trim-NHLs that associate with miRNA pathway proteins. So far, two have been shown to repress (Mei-P26 and Lin41) and two to promote (NHL-2, Trim32) miRNA-mediated gene silencing. In this chapter we will describe structure-function relations for each of the proteins and then focus on the lessons being learned from these proteins about miRNA functions in development and in stem cell biology.

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  1. Center for Anatomy, Institute of Cell Biology and Neurobiology, Charité, Universitätsmedizin Berlin, Berlin, Germany

    F. Gregory Wulczyn

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  2. Elisa Cuevas
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  3. Eleonora Franzoni
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  4. Agnieszka Rybak
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  1. Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

    Helge Großhans

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Wulczyn, F.G., Cuevas, E., Franzoni, E., Rybak, A. (2010). miRNAs Need a Trim. In: Großhans, H. (eds) Regulation of microRNAs. Advances in Experimental Medicine and Biology, vol 700. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7823-3_9

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