Abstract
Objective: To research the 3′-daidzein sulfonic sodium’s (DSS) effect on anti-oxidation of retinal ischemia/reperfusion (RI/R) injury in rats.
Methods: RI/R in rats was made by reperfusion for 1 h after occlusion of common carotid artery (CCA) for 1 h and the rats were divided into four groups randomly, including sham control group, model group of RI/R injury, low-dosage DSS group and high-dosage DSS group. 1 ml/kg NS was injected through sublingual vein after CCA was dissociated in sham group. Other groups were treated with normal saline, low-dosage DSS (1 mg/kg) and high-dosage DSS (2 mg/kg) through sublingual vein respectively before occlusion of CCA. After occlusion of CCA for 1 h and reperfusion for 1 h, blood was put out and separated into the serum. Then detect the contents of MDA and NO, the activity of SOD and GSH-PX.
Result: (1) The content of MDA was increased in model group (P<0.05), and low-dosage DSS can reverse it (P<0.05). (2) The content of serum NO in model group was lower than in sham group (P<0.001). The content of serum NO in model group is lower than in low-dosage DSS group (P<0.05) and high-dosage DSS group (P<0.01) respectively. (3) The activity of GSH-PX in high-dosage group was higher than in model group (P<0.05). (4) The activity of SOD in low-dosage group was higher than in model group (P<0.05).
Conclusion: DSS can decrease the cellular damage and protect the optical function in RI/R injury through elevating the content of NO, the activity of SOD and GSH-PX, and increasing anti-oxidation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Aydemir O, Naziroglu M, Celebi S et al (2004) Antioxidant effects of alpha-, gamma- and succinate – tocopherols in guinea pig retina during ischemia-reperfusion injury. Pathophysiology 11:167–171
Li L, Zeng J, Qiu F (2005) Effect of 3′-Daidzein sulfonate sodium on contractle of rabbit thoracic aortic strips. Drug Dev Res 66:243
Neal MJ, Cunningham JR, Hutson PH et al (1994) Effects of ischaemia on neurotransmitter release from the isolated retina. J Neurochem 62:1025–1033
Ophir A, Berenshtein E, Kitrossky N et al (1993) Hydroxyl radical generation in the cat retina during reperfusion following ischemia. Exp Eye Res 57:351–357
Pannicke T, Uckermann O, Iandiev I et al (2005) Altered membrane physiology in Muller glial cells after transient ischemia of the rat retina. Glia 50:1–11
Peachey NS, Green DJ, Ripps H (1993) Ocular ischemia and the effects of allopurinol on functional recovery in the retina of the arterially perfused cat eye. Invest Ophthalmol Vis Sci 34:58–65
Rios L, Cluzel J, Vennat JC et al (1999) Comparison of intraocular treatment of DMTU and SOD following retinal ischemia in rats. Ocul Pharmacol Ther 15:547–556
Romano C, Price M, Bai HY et al (1993) Neuroprotectants in Honghua: glucose attenuates retinal ischemic damage. Invest Ophthalmol Vis Sci 34:72–80
Song Y, Gong YY, Xie ZG et al (2008) Edaravone (MCI-186), a free radical scavenger, attenuates retinal ischemia/reperfusion injury in rats. Acta Pharmacol Sin 29:823–828
Szabo ME, Droy-Lefaix MT, Doly M et al (1991) Free radical-mediated effects in reperfusion injury: a histologic study with superoxyde dismutase and Egb 761 in rat retina. Ophthalmic Res 23:225–234
Szabo ME, Droy-Lefaix MT, Doly M (1997) Direct measurement of free radicals in ischemic/reperfused diabetic rat retina. Clin Neurosci 4:240–245
Tsujikawa A, Ogura Y, Hiroshiba N et al (1999) Retinal ischemia-reperfusion injury attenuated by blocking of adhesion molecules of vascular endothelium. Invest Ophthalmol Vis Sci 40:1183–1190
Veriac S, Tissie G, Bonne C (1993) Oxygen free radicals adversely affect the regulation of vascular tone by nitric oxide in the rabbit retina under high intraocular pressure. Exp Eye Res 56:85–88
Zeng J, Zeng Z, Huang Z et al (2005) Anti-hypoxia effect of 3′-daidzein. J Clin Rehabil 10:130–132
Acknowledgments
This work was supported by National Nature Science Fund (NO.30760284); Department of Education of Jiangxi Province (NO.GJJ08394); Department of Health Care of Jiangxi Province (NO.20072034).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Zhihua, H., Liangdong, L., Xiao, L., Fang, C., Jing, Z. (2010). Effect of 3′-Daidzein Sulfonic Sodium on the Anti-oxidation of Retinal Ischemia/Reperfusion Injury in Rats. In: Anderson, R., Hollyfield, J., LaVail, M. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 664. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1399-9_67
Download citation
DOI: https://doi.org/10.1007/978-1-4419-1399-9_67
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4419-1398-2
Online ISBN: 978-1-4419-1399-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)