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Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma

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Patient-Derived Xenografts

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2806))

Abstract

Colorectal cancer (CRC) is a significant global health concern, requiring effective preclinical models for studying its development and testing therapies. Mouse models have been used, including spontaneous tumors, carcinogen exposure, and tumor cell implantation as xenografts or at orthotopic sites. Here, we describe an orthotopic preclinical model of CRC, which provides a valuable tool for studying tumor growth and the tumor microenvironment, offering a more accurate representation of human CRC compared to xenograft models.

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References

  1. Keum N, Giovannucci E (2019) Global burden of colorectal cancer: emerging trends, risk factors and prevention strategies. Nat Rev Gastroenterol Hepatol 16(12):713–732

    Article  PubMed  Google Scholar 

  2. Moser AR, Pitot HC, Dove WF (1990) A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse. Science 247(4940):322–324

    Article  CAS  PubMed  Google Scholar 

  3. Reitmair AH, Schmits R, Ewel A, Bapat B et al (1995) MSH2 deficient mice are viable and susceptible to lymphoid tumours. Nat Genet 11(1):64–70. https://doi.org/10.1038/ng0995-64

    Article  CAS  PubMed  Google Scholar 

  4. Su L-K, Kinzler KW, Vogelstein B et al (1992) Multiple intestinal neoplasia caused by a mutation in the murine homolog of the APC gene. Science 256(5057):668–670

    Article  CAS  PubMed  Google Scholar 

  5. Tanaka T, Kohno H, Suzuki R et al (2003) A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate. Cancer Sci 94(11):965–973

    Article  CAS  PubMed  Google Scholar 

  6. Wilmanns C, Fan D, O'Brian CA et al (1992) Orthotopic and ectopic organ environments differentially influence the sensitivity of murine colon carcinoma cells to doxorubicin and 5-fluorouracil. Int J Cancer 52(1):98–104. https://doi.org/10.1002/ijc.2910520118

    Article  CAS  PubMed  Google Scholar 

  7. Chen C, Neumann J, Kühn F, et al (2020) Establishment of an Endoscopy-Guided Minimally Invasive Orthotopic Mouse Model of Colorectal Cancer. Cancers (Basel) 12(10):3007. https://doi.org/10.3390/cancers12103007

  8. Tan MH, Holyoke ED, Goldrosen MH (1977) Murine colon adenocarcinoma: syngeneic orthotopic transplantation and subsequent hepatic metastases. J Natl Cancer Inst 59(5):1537–1544. https://doi.org/10.1093/jnci/59.5.1537

    Article  CAS  PubMed  Google Scholar 

  9. Alencar H, King R, Funovics M et al (2005) A novel mouse model for segmental orthotopic colon cancer. Int J Cancer 117(3):335–339

    Article  CAS  PubMed  Google Scholar 

  10. Céspedes MV, Espina C, García-Cabezas MA et al (2007) Orthotopic microinjection of human colon cancer cells in nude mice induces tumor foci in all clinically relevant metastatic sites. Am J Pathol 170(3):1077–1085. https://doi.org/10.2353/ajpath.2007.060773

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Yakou MH, Ghilas S, Tran K, et al (2023) TCF-1 limits intraepithelial lymphocyte antitumor immunity in colorectal carcinoma. Sci Immunol 8(88): https://doi.org/10.1126/sciimmunol.adf2163

  12. Jacobse J, Pilat JM, Li J et al (2024) Distinct roles for interleukin-23 receptor signaling in regulatory T cells in sporadic and inflammation-associated carcinogenesis. Front. oncol 13 (1276743). https://doi.org/10.3389/fonc.2023.1276743

  13. Zhao X, Li L, Starr TK et al (2017) Tumor location impacts immune response in mouse models of colon cancer. Oncotarget 8(33):54775–54787. https://doi.org/10.18632/oncotarget.18423

    Article  PubMed  PubMed Central  Google Scholar 

  14. Selby MJ, Engelhardt JJ, Johnston RJ et al (2016) Preclinical development of ipilimumab and nivolumab combination immunotherapy: mouse tumor models, in vitro functional studies, and cynomolgus macaque toxicology. PLoS One 11(9):e0161779. https://doi.org/10.1371/journal.pone.0161779

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, Australia

    Marina H. Yakou, Shoukat Afshar-Sterle, Matthias Ernst & Lisa A. Mielke

Authors
  1. Marina H. Yakou
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  2. Shoukat Afshar-Sterle
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  3. Matthias Ernst
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  4. Lisa A. Mielke
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Corresponding authors

Correspondence to Marina H. Yakou , Matthias Ernst or Lisa A. Mielke .

Editor information

Editors and Affiliations

  1. South Australian immunoGENomics Cancer Institute (SAiGENCI), University of Adelaide, Adelaide, SA, Australia

    Mohamed I. Saad

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yakou, M.H., Afshar-Sterle, S., Ernst, M., Mielke, L.A. (2024). Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma. In: Saad, M.I. (eds) Patient-Derived Xenografts. Methods in Molecular Biology, vol 2806. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3858-3_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3858-3_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3857-6

  • Online ISBN: 978-1-0716-3858-3

  • eBook Packages: Springer Protocols

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