Abstract
High ambient temperature affects various plant developmental and physiological processes, including senescence. Here, we present a protocol for assaying light-dependent high ambient temperature-induced senescence using whole seedlings. The protocol covers all steps, from inducing senescence by darkness at high ambient temperature to determining the degree of senescence, and includes experimental tips and notes. The onset of senescence is established by quantifying the increased expression of senescence marker genes by quantitative real-time PCR (RT-qPCR). The degree of senescence is determined by measuring the loss of chlorophyll and the increase of ion leakage. This protocol can be adapted to study light-dependent high ambient temperature-induced senescence in other plant species by adjusting the temperature and duration of darkness.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Kim, C., Choi, G. (2024). Light-Dependent High Ambient Temperature-Induced Senescence Assay Using Whole Seedlings. In: Chen, M. (eds) Thermomorphogenesis. Methods in Molecular Biology, vol 2795. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3814-9_3
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DOI: https://doi.org/10.1007/978-1-0716-3814-9_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3813-2
Online ISBN: 978-1-0716-3814-9
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