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Measurement of Algal Photosynthesis Using a Clark-Type O2 Electrode

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Photosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2790))

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Abstract

The Clark-type electrode can be used to assess the rates of photosynthesis by detecting changes in O2 concentration in a culture. This chapter describes a method for a liquid phase measurement of light and dissolved inorganic carbon-dependent photosynthesis using the model green alga Chlamydomonas reinhardtii. The technique can be used to evaluate the presence or efficiency of carbon-concentrating mechanisms.

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Acknowledgments

We would like to thank Dr. Kher Xing Chan for her assistance in establishing the procedure in the laboratory and Dr. Jim Moroney for advice, tips, and tricks on how to obtain optimal results.

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Authors and Affiliations

  1. Department of Plant Biology and Carl R Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL, USA

    Steven J. Burgess & Chandra Davies

Authors
  1. Steven J. Burgess
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  2. Chandra Davies
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Corresponding author

Correspondence to Steven J. Burgess .

Editor information

Editors and Affiliations

  1. Colorado Springs, CO, USA

    Sarah Covshoff

1 Electronic Supplementary Materials

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Burgess, S.J., Davies, C. (2024). Measurement of Algal Photosynthesis Using a Clark-Type O2 Electrode. In: Covshoff, S. (eds) Photosynthesis . Methods in Molecular Biology, vol 2790. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3790-6_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3790-6_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3789-0

  • Online ISBN: 978-1-0716-3790-6

  • eBook Packages: Springer Protocols

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