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Understanding Actin Remodeling in Neuronal Cells Through Podosomes

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Neuroprotection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2761))

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Abstract

Cytoskeletal dysregulation forms an important aspect of many neurodegenerative diseases such as Alzheimer’s disease. Cytoskeletal functions require the dynamic activity of the cytoskeletal proteins—actin, tubulin, and the associated proteins. One of such important phenomena is that of actin remodeling, which helps the cell to migrate, navigate, and interact with extracellular materials. Podosomes are complex actin-rich cytoskeletal structures, abundant in proteins that interact and degrade the extracellular matrix, enabling cells to displace and migrate. The formation of podosomes requires extensive actin networks and remodeling. Here we present a novel immunofluorescence-based approach to study actin remodeling in neurons through the medium of podosomes.

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Acknowledgments

This project is supported by the in-house CSIR-National Chemical Laboratory grant MLP101726. The author is grateful to Chinnathambi’s lab members for their scientific suggestions on the manuscript. TQ acknowledges the Department of Science and Technology – Innovation in Science Pursuit for Inspired Research (DST-INSPIRE), the Government of India for her fellowship. The authors greatly acknowledge the Department of Neurochemistry, the National Institute of Mental Health and Neuro Sciences (NIMHANS), and the Institute of National Importance, Bangalore, for their internal support.

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Authors and Affiliations

  1. Neurobiology Group, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India

    Tazeen Qureshi, Smita Eknath Desale, Hariharakrishnan Chidambaram & Subashchandrabose Chinnathambi

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India

    Tazeen Qureshi, Smita Eknath Desale, Hariharakrishnan Chidambaram & Subashchandrabose Chinnathambi

  3. Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences (NIMHANS), Institute of National Importance, Bangalore, Karnataka, India

    Tazeen Qureshi, Smita Eknath Desale, Hariharakrishnan Chidambaram & Subashchandrabose Chinnathambi

Authors
  1. Tazeen Qureshi
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  2. Smita Eknath Desale
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  3. Hariharakrishnan Chidambaram
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  4. Subashchandrabose Chinnathambi
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Correspondence to Subashchandrabose Chinnathambi .

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Editors and Affiliations

  1. Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA

    Swapan K. Ray

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Qureshi, T., Desale, S.E., Chidambaram, H., Chinnathambi, S. (2024). Understanding Actin Remodeling in Neuronal Cells Through Podosomes. In: Ray, S.K. (eds) Neuroprotection. Methods in Molecular Biology, vol 2761. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3662-6_18

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  • DOI: https://doi.org/10.1007/978-1-0716-3662-6_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3661-9

  • Online ISBN: 978-1-0716-3662-6

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