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Rearing and Conservation of Nematode Populations Under Greenhouse or Axenic Conditions

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Plant-Nematode Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2756))

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Abstract

Once a nematode has been identified, to conduct studies for screening programs or pathogenicity tests, it is necessary a supply of large numbers of nematodes from field crops or reproduced and stored to be used in periods of the year when they are not available from fields. Therefore, nematodes must be reared in greenhouse or under in vitro conditions and stored for future needs. In this chapter, suggestions are given on how to obtain nematodes from fields and reproduce most of them on host plants in greenhouse (mainly Meloidogyne spp. and Globodera spp.) or in vitro. Reproductions in vitro include:

  • On suitable callus of host plants (Pratylenchus spp., Ditylenchus spp.)

  • On fungal cultures mainly of Botrytis cinerea or Alternaria spp. for Aphelenchoides spp. and other aphelenchids, including Bursaphelenchus xylophilus.

  • On carrot disks for Pratylenchus spp. and Ditylenchus spp.

  • Other specific media, such as garlic, potato, and sweet potato for D. destructor, and cocoyam disks for Radopholus similis.

Guidelines are also given to store different nematodes for rather long times, including in vitro methods and in infected seeds, hay, and other plant parts. No information is given on how to prepare and store fixed materials.

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Authors and Affiliations

  1. Institute for Sustainable Plant Protection, National Research Council of Italy (CNR), Bari, Italy

    Nicola Greco & Alberto Troccoli

Authors
  1. Nicola Greco
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  2. Alberto Troccoli
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Editors and Affiliations

  1. IPSP, Consiglio Nazionale delle Ricerche CNR, Bari, Italy

    Sergio Molinari

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Greco, N., Troccoli, A. (2024). Rearing and Conservation of Nematode Populations Under Greenhouse or Axenic Conditions. In: Molinari, S. (eds) Plant-Nematode Interactions. Methods in Molecular Biology, vol 2756. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3638-1_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3638-1_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3637-4

  • Online ISBN: 978-1-0716-3638-1

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