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Protocol of Geometric Morphometrics for Teratogenicity Testing

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Teratogenicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2753))

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Abstract

Geometric morphometrics (GM) enables a quantitative study of shapes and forms allowing the identification and characterization of teratogenic malformations. The GM methodology offers several advantages in comparison to traditional biometric methods, such as higher detail and precision analysis. In this chapter, we describe the recent application of the Procrustes method with ImageJ and MorphoJ programs in the characterization of developmental malformations. With this methodology, we are a step closer to being able to assign molecular pathways or unique signatures to a specific teratogen according to the produced phenotypes or to cluster unknown compounds.

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Acknowledgments

This work is supported by National Funds from FCT – Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020, and Junior Researcher contract 2021.00458.CEECIND (Luís Félix).

Author information

Authors and Affiliations

  1. Animal and Veterinary Department, University Institute of Health Sciences, CESPU, CRL, Gandra, Portugal

    Mariana Caipira Lei

  2. Center for the Research and Technology of Agro-Environment and Biological Sciences (CITAB), UTAD, Vila Real, Portugal

    Luís Félix & Carlos Venâncio

  3. Institute for Innovation, Capacity Building and Sustainability of Agri-food Production (Inov4Agro), UTAD, Vila Real, Portugal

    Luís Félix & Carlos Venâncio

  4. Department of Animal Science, School of Agrarian and Veterinary Sciences (ECAV), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal

    Carlos Venâncio

Authors
  1. Mariana Caipira Lei
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  2. Luís Félix
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  3. Carlos Venâncio
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Corresponding author

Correspondence to Mariana Caipira Lei .

Editor information

Editors and Affiliations

  1. CITAB, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal

    Luís Félix

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Lei, M.C., Félix, L., Venâncio, C. (2024). Protocol of Geometric Morphometrics for Teratogenicity Testing. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 2753. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3625-1_28

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  • DOI: https://doi.org/10.1007/978-1-0716-3625-1_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3624-4

  • Online ISBN: 978-1-0716-3625-1

  • eBook Packages: Springer Protocols

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