Abstract
DNA barcoding employs standard molecular techniques (e.g., DNA extraction, PCR, and Sanger sequencing) to taxonomically identify biological samples. While DNA barcoding is a useful experimental workflow for in-class active learning exercises, extracting DNA from diverse sample types in a time and cost-effective manner can be challenging in a classroom setting. Here, we provide two time and cost-effective methods that have been used by novice students to successfully extract DNA from a variety of animal, fungal, algal, and plant tissues for DNA barcoding.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Hyman, O., Cass, A., Enke, R., Storm, A., Nash, B. (2024). Cost-Effective DNA Extraction for DNA Barcoding Diverse Biological Samples. In: DeSalle, R. (eds) DNA Barcoding. Methods in Molecular Biology, vol 2744. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3581-0_7
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DOI: https://doi.org/10.1007/978-1-0716-3581-0_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3580-3
Online ISBN: 978-1-0716-3581-0
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