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RNA Extraction from Gram-Positive Bacteria Membrane Vesicles Using a Polymer-Based Precipitation Method

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Bacterial Regulatory RNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2741))

Abstract

Investigations into the biological role and composition of bacterial extracellular vesicles have grown in popularity in recent years. Vesicles perform a variety of functions during interactions with eukaryotic host cells, ranging from antibiotic resistance to immune modulation. It is necessary to isolate vesicles in order to understand their biological functions. Here we describe a polymer-based precipitation method allowing high-yield isolation of extracellular vesicles and their cargo RNA from the Gram-positive bacterium Staphylococcus aureus.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Ohio University, Athens, OH, USA

    Paul Briaud & Ronan K. Carroll

  2. Molecular and Cellular Biology Program, Ohio University, Athens, OH, USA

    Ronan K. Carroll

  3. Infectious and Tropical Disease Institute, Ohio University, Athens, OH, USA

    Ronan K. Carroll

Authors
  1. Paul Briaud
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  2. Ronan K. Carroll
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Corresponding author

Correspondence to Paul Briaud .

Editor information

Editors and Affiliations

  1. CEA Saclay, Lab Léon Brillouin UMR12 CEA/CNRS, Gif-sur-Yvette, France

    Véronique Arluison

  2. Laboratorio de Fisiología y Genética de Bacterias Beneficiosas para Plantas (LFGBBP), Centro de Bioquímica y Microbiología del Suelo (CBMS), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes – CONICET, Bernal, Buenos Aires, Argentina

    Claudio Valverde

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Briaud, P., Carroll, R.K. (2024). RNA Extraction from Gram-Positive Bacteria Membrane Vesicles Using a Polymer-Based Precipitation Method. In: Arluison, V., Valverde, C. (eds) Bacterial Regulatory RNA. Methods in Molecular Biology, vol 2741. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3565-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3565-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3564-3

  • Online ISBN: 978-1-0716-3565-0

  • eBook Packages: Springer Protocols

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