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Development of Organoids to Study Infectious Host Interactions

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Borrelia burgdorferi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2742))

Abstract

Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.

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Authors and Affiliations

  1. Max Planck Institute of Molecular Biology and Genetics, Dresden, Germany

    Aparajita Lahree

  2. Tezted Ltd, Jyväskylä, Finland

    Leona Gilbert

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  1. Tezted Ltd, Jyväskylä, Finland

    Leona Gilbert

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Lahree, A., Gilbert, L. (2024). Development of Organoids to Study Infectious Host Interactions. In: Gilbert, L. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 2742. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3561-2_12

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  • DOI: https://doi.org/10.1007/978-1-0716-3561-2_12

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