Abstract
The protein–protein interactions (PPI) by protein array technology complement other PPI assay technologies such as AP-MS and Y2H. The in situ protein array technology (NAPPA) enables low-cost, rapid, and comprehensive protein detection. It allows standardized and simultaneous assay of a wide range of proteins with a broad range of expression in cells. This technology facilitates the detection of protein–protein interactions within species and between heterologous species such as host–microbe. Here, we described the technique that identified a syntaxin-6 protein-mediated begomovirus infection using an array containing 4600 Arabidopsis genes. The protein microarray assay also identified several other viral protein–host protein interactions.
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Acknowledgments
This protocol has been derived and adapted from Yazaki et al. [6, 7] and Gouveia-Mageste et al. [8]. This work was supported partially by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (C), 2019-2021 [19 K0574 to J.Y.]. The work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico [220164/2012-6 and 312641/2021-4 to MD), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (APQ-01416-16, PPM-640-18 and RED-00124-22 to MD), and Caramuru Alimentos (Grant to MD). We would like to thank Editage (www.editage.com) for English language editing.
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Yazaki, J., Dal-Bianco, M. (2024). In Situ Protein Microarray for Identifying the Geminivirus–Arabidopsis Interactome. In: Fontes, E.P., Mäkinen, K. (eds) Plant-Virus Interactions. Methods in Molecular Biology, vol 2724. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3485-1_22
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DOI: https://doi.org/10.1007/978-1-0716-3485-1_22
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