Abstract
The ability of Pseudomonas aeruginosa to establish chronic infections is associated with an effective switch from a motile to a sessile lifestyle. This proficiency is controlled by intracellular levels of the second messenger bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP). Targeting the c-di-GMP network could be a strategy to interfere with P. aeruginosa pathogenicity. Therefore, the development of tools to profile c-di-GMP intracellular levels is crucial. Here, we describe a protocol for the in vivo measurement of c-di-GMP levels in P. aeruginosa.
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Acknowledgments
We are grateful to Livia Leoni and Giordano Rampioni for providing the P. aeruginosa strains used in this work. This work has been supported by the European Commission (NABATIVI-223670 and EU-FP7-HEALTH-2007-B) and Italian Cystic Fibrosis Research Foundation (FFC#13/2015) with the contribution of Gruppo di Sostegno FFC di Sassari Castelsardo and Delegazione FFC di Boschi Sant’Anna Minerbe and (FFC#14/2016) with the contribution of Delegazione FFC di Reggio Calabria and Gruppo di Sostegno FFC di Vigevano.
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Santoro, S., Bertoni, G., Ferrara, S. (2024). Fluorescence-based Evaluation of Cyclic di-GMP Levels in Pseudomonas aeruginosa. In: Bertoni, G., Ferrara, S. (eds) Pseudomonas aeruginosa. Methods in Molecular Biology, vol 2721. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3473-8_4
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DOI: https://doi.org/10.1007/978-1-0716-3473-8_4
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