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Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy

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Food Allergens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2717))

Abstract

As allergies, especially those triggered by food, are becoming more and more prevalent, it is of increasing importance to fully understand the structures and dynamic behaviors of allergenic proteins along with their interactions with potential natural ligands. Therefore, we have established a solid routine to achieve structural characterization of food allergens, especially for birch pollen-related cross-reactive proteins from the class 10 of pathogenesis-related proteins (PR-10), by nuclear magnetic resonance (NMR) spectroscopy. Following expression of the desired allergen in Escherichia coli in isotope-labeled minimal media, the three-dimensional solution structures of these proteins can be determined, and insight into ligand binding mechanics and structural dynamic properties are accessible through NMR spin relaxation experiments.

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Authors and Affiliations

  1. Institute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria

    Ricarda Zeindl, Jana Unterhauser, Manuel Röck, Reiner Eidelpes, Sebastian Führer & Martin Tollinger

Authors
  1. Ricarda Zeindl
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  2. Jana Unterhauser
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  3. Manuel Röck
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  4. Reiner Eidelpes
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  5. Sebastian Führer
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  6. Martin Tollinger
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Corresponding author

Correspondence to Martin Tollinger .

Editor information

Editors and Affiliations

  1. Department of Allergy, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain

    Beatriz Cabanillas

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zeindl, R., Unterhauser, J., Röck, M., Eidelpes, R., Führer, S., Tollinger, M. (2024). Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy. In: Cabanillas, B. (eds) Food Allergens. Methods in Molecular Biology, vol 2717. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3453-0_10

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  • DOI: https://doi.org/10.1007/978-1-0716-3453-0_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3452-3

  • Online ISBN: 978-1-0716-3453-0

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