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Components Subcellular Localization: Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

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Bacterial Secretion Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2715))

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Abstract

Bacterial lipoproteins are characterized by fatty acids, derived from membrane phospholipids, which are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Here, I describe the detection of one of the intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using 3H-palmitate labeling and inhibition of signal peptidase II (Lsp) by globomycin and detection by fluorography.

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Authors and Affiliations

  1. Institut Pasteur, Université Paris Cité, CNRS UMR6047, INSERM U1306, Biology and genetics of the bacterial cell wall Unit, 25-28 rue du docteur Roux, Paris cedex 15, France

    Nienke Buddelmeijer

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  1. Nienke Buddelmeijer
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Correspondence to Nienke Buddelmeijer .

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Editors and Affiliations

  1. Laboratoire d’Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ, CNRS, Marseille, France

    Laure Journet

  2. Laboratoire d’Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ, CNRS, Marseille, France

    Eric Cascales

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Buddelmeijer, N. (2024). Components Subcellular Localization: Identification of Lipoproteins Using Globomycin and Radioactive Palmitate. In: Journet, L., Cascales, E. (eds) Bacterial Secretion Systems . Methods in Molecular Biology, vol 2715. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3445-5_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3445-5_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3444-8

  • Online ISBN: 978-1-0716-3445-5

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