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Techniques in Staining of Rodent and Human Olfactory Tissue

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The Olfactory System

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2710))

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Abstract

Staining of olfactory tissue allows for evaluation of the organization of specific cell types within the specimen and allows for assessment in abnormalities of function that may be related to changes in normal tissue architecture. Histochemical staining and immunohistochemical (IHC) techniques are the most common methods for visualizing olfactory epithelium and olfactory bulbs. Here we describe the method of IHC for olfactory tissue utilizing both fluorescent and peroxidase-based methods of visualization.

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Author information

Authors and Affiliations

  1. Department of Otolaryngology—Head and Neck Surgery, The University of Tokyo, Tokyo, Japan

    Hironobu Nishijima

  2. Department of Otolaryngology—Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, USA

    Eric H. Holbrook

Authors
  1. Hironobu Nishijima
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  2. Eric H. Holbrook
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Corresponding author

Correspondence to Eric H. Holbrook .

Editor information

Editors and Affiliations

  1. Department of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC, USA

    Bradley J. Goldstein

  2. Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA

    Hiroaki Matsunami

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Nishijima, H., Holbrook, E.H. (2023). Techniques in Staining of Rodent and Human Olfactory Tissue. In: Goldstein, B.J., Matsunami, H. (eds) The Olfactory System. Methods in Molecular Biology, vol 2710. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3425-7_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3425-7_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3424-0

  • Online ISBN: 978-1-0716-3425-7

  • eBook Packages: Springer Protocols

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