Abstract
The use of Hox-driven conditionally immortalized immune cells has significantly increased in biomedical research over the past 15 years. HoxB8-driven conditionally immortalized myeloid progenitor cells maintain their ability to differentiate into functional macrophages. There are multiple benefits to this conditional immortalization strategy including the ability for unlimited propagation, genetic mutability, primary-like immune cells (macrophages, dendritic cells, and granulocytes) on demand, derivation from variety of mouse strains, and simple cryopreservation and reconstitution. In this chapter, we will discuss how to derive and use these HoxB8-conditionally immortalized myeloid progenitor cells.
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References
Kondo M (2010) Lymphoid and myeloid lineage commitment in multipotent hematopoietic progenitors. Immunol Rev 238:37–46
Wang GG, Calvo KR, Pasillas MP, Sykes DB, Häcker H, Kamps MP (2006) Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8. Nat Methods 3:287–293
Odegaard JI, Vats D, Zhang L, Ricardo-Gonzalez R, Smith KL, Sykes DB, Kamps MP, Chawla A (2007) Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. J Leukoc Biol 81:711–719
Lail SS, Arnold CR, de Almeida LG, McKenna N, Chiriboga JA, Dufour A, Warren AL, Yates RM (2022) Hox-driven conditional immortalization of myeloid and lymphoid progenitors: uses, advantages, and future potential. Traffic 23:538–553
Ramezani A, Hawley TS, Hawley RG (2000) Lentiviral vectors for enhanced gene expression in human hematopoietic cells. Mol Ther 2:458–469
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Lail, S.S., McKenna, N., Yates, R.M. (2023). The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages. In: Botelho, R.J. (eds) Phagocytosis and Phagosomes. Methods in Molecular Biology, vol 2692. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3338-0_8
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DOI: https://doi.org/10.1007/978-1-0716-3338-0_8
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