Skip to main content
SpringerLink
Log in

Protein-Protein Interactions

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Shahid Mukhtar

    1. Department of Biology, University of Alabama at Birmingham, Birmingham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2690)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (36 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Yeast Two-Hybrid Technique to Identify Protein–Protein Interactions

    • Prabu Gnanasekaran, Hanu R. Pappu
    Pages 1-8

  3. Cytotrap: An Innovative Approach for Protein–Protein Interaction Studies for Cytoplasmic Proteins

    • Binoop Mohan, Doni Thingujam, Karolina M. Pajerowska-Mukhtar
    Pages 9-22

  4. Analyzing Protein–Protein Interactions Using the Split-Ubiquitin System

    • Rucha Karnik, Michael R. Blatt
    Pages 23-36

  5. Detection of Protein–Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System

    • Siddhartha Dutta, Matthew D. Smith
    Pages 37-57

  6. Preparation and Utilization of a Versatile GFP-Protein Trap-Like System for Protein Complex Immunoprecipitation in Plants

    • Danish Diwan, Karolina M. Pajerowska-Mukhtar
    Pages 59-68

  7. Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins

    • Teck Yew Low, Pey Yee Lee
    Pages 69-80

  8. Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein–Protein Interactions

    • Prabu Gnanasekaran, Hanu R. Pappu
    Pages 81-85

  9. Co-immunoprecipitation-Based Identification of Effector–Host Protein Interactions from Pathogen-Infected Plant Tissue

    • Mamoona Khan, Armin Djamei
    Pages 87-100

  10. Co-immunoprecipitation for Assessing Protein–Protein Interactions in Agrobacterium-Mediated Transient Expression System in Nicotiana benthamiana

    • Ji Chul Nam, Padam S. Bhatt, Sung-Il Kim, Hong-Gu Kang
    Pages 101-110

  11. Detection of Protein–Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique

    • Prabu Gnanasekaran, Hanu R. Pappu
    Pages 111-115

  12. Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein–Protein Interactions in Living Cells

    • Prabu Gnanasekaran, Hanu R. Pappu
    Pages 117-120

  13. Detecting Protein–Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA)

    • Pepijn Bais, Louai Alidrissi, Ikram Blilou
    Pages 121-131

  14. Forster Resonance Energy Transfer (FRET) to Visualize Protein–Protein Interactions in the Plant Cell

    • Prabu Gnanasekaran, Hanu R. Pappu
    Pages 133-135

  15. Dynamic Proximity Tagging in Living Plant Cells with Pupylation-Based Interaction Tagging

    • Ruiqiang Ye, Zhuoran Lin, Kun-Hsaing Liu, Jen Sheen, Sixue Chen
    Pages 137-147

  16. Characterization of Small Molecule–Protein Interactions Using SPR Method

    • Binmei Sun, Jianmei Xu, Shaoqun Liu, Qing X. Li
    Pages 149-159

  17. High-Throughput Protein–Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline

    • Benoît Castandet, Claire Lurin, Étienne Delannoy, Dario Monachello
    Pages 161-177

  18. Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two-Hybrid (Y2H) Protocol to Evaluate Networks

    • Ali Zeeshan Fakhar, Jinbao Liu, Karolina M. Pajerowska-Mukhtar
    Pages 179-192

  19. An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases

    • Jente Stouthamer, Sergio Martin-Ramirez, Elwira Smakowska-Luzan
    Pages 193-204

  20. Next-Generation Yeast Two-Hybrid Screening to Discover Protein–Protein Interactions

    • J. Mitch Elmore, Valeria Velásquez-Zapata, Roger P. Wise
    Pages 205-222
Back to top

Keywords

About this book

This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Protein-Protein Interactions: Methods and Protocols serves as an ideal guide for researchers working in protein science and beyond.

Editors and Affiliations

  • Department of Biology, University of Alabama at Birmingham, Birmingham, USA

    Shahid Mukhtar

Bibliographic Information

  • Book Title: Protein-Protein Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Shahid Mukhtar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3327-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3326-7Published: 15 July 2023

  • Softcover ISBN: 978-1-0716-3329-8Due: 22 September 2023

  • eBook ISBN: 978-1-0716-3327-4Published: 14 July 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 486

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Protein Science, Chemistry/Food Science, general

Publish with us

Policies and ethics

Back to top

Search

Navigation