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Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells

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Stem Cell-Based Neural Model Systems for Brain Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2683))

Abstract

Oligodendrocyte progenitor cells (OPCs) and mature oligodendrocytes (OLs) can be generated using human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs). By manipulating culture conditions, pluripotent cell types are serially guided through intermediary cell types, developing first into neural progenitor cells (NPCs) then OPCs before maturing as CNS-specific OLs. This procedure is conducted under adherent, feeder-free conditions to derive mature OLs in as few as 28 days.

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Author information

Authors and Affiliations

  1. Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA

    Zoe Mattingly & Sundari Chetty

  2. Department of Psychiatry, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

    Sundari Chetty

  3. Harvard Stem Cell Institute, Cambridge, MA, USA

    Sundari Chetty

Authors
  1. Zoe Mattingly
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  2. Sundari Chetty
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Corresponding author

Correspondence to Sundari Chetty .

Editor information

Editors and Affiliations

  1. Department of Molecular Biology, Cell Biology, and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA

    Yu-Wen Alvin Huang

  2. Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA

    ChangHui Pak

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mattingly, Z., Chetty, S. (2023). Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells. In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3287-1_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3287-1_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3286-4

  • Online ISBN: 978-1-0716-3287-1

  • eBook Packages: Springer Protocols

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