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Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE

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Diabetic Retinopathy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2678))

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Abstract

In vitro generation of a functional retinal pigment epithelium (RPE) monolayer sheet is useful and promising for RPE cell therapy. Here, we outline a method to construct engineered RPE sheets treated by induced pluripotent stem cell-conditioned medium (iPS-CM) in conjunction with femtosecond laser intrastromal lenticule (FLI-lenticule) scaffold to aid in enhanced RPE characteristics and cilium assembly. Such a strategy to construct RPE sheets is a promising avenue for developing RPE cell therapy, disease models, and drug screening tools.

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Author information

Authors and Affiliations

  1. Aier Eye Institute, Changsha, Hunan Province, People’s Republic of China

    Jianing Gu, Yini Wang, Chengcheng Ding, Shibo Tang & Jiansu Chen

  2. Aier School of Ophthalmology, Central South University, Changsha, Hunan Province, People’s Republic of China

    Yini Wang, Shibo Tang & Jiansu Chen

  3. Changsha Aier Eye Hospital, Changsha, Hunan Province, People’s Republic of China

    Shibo Tang & Jiansu Chen

Authors
  1. Jianing Gu
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  2. Yini Wang
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  3. Chengcheng Ding
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  4. Shibo Tang
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  5. Jiansu Chen
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Corresponding authors

Correspondence to Shibo Tang or Jiansu Chen .

Editor information

Editors and Affiliations

  1. Centre for Eye Research Australia, University of Melbourne, East Melbourne, VIC, Australia

    Guei-Sheung Liu

  2. Centre for Eye Research Australia, University of Melbourne, East Melbourne, VIC, Australia

    Jiang-Hui Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gu, J., Wang, Y., Ding, C., Tang, S., Chen, J. (2023). Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE. In: Liu, GS., Wang, JH. (eds) Diabetic Retinopathy. Methods in Molecular Biology, vol 2678. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3255-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3255-0_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3254-3

  • Online ISBN: 978-1-0716-3255-0

  • eBook Packages: Springer Protocols

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