Abstract
The molecular basis of Z-DNA recognition and stabilization is mostly discovered via X-ray crystallography. The sequences composed with alteration of purine and pyrimidine are known to adopt Z-DNA conformation. Due to the energy penalty for forming Z-DNA, the small molecular stabilizer or Z-DNA-specific binding protein is required for DNA to adopt Z conformation prior to crystallizing Z-DNA. Here we described the methods ranging from preparation of DNA and Z-alpha protein to crystallization of Z-DNA in detail.
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Acknowledgments
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science, and Technology (MSIT) of Korean government (2020RC1C1C1007371 to D.K.).
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Kang, M., Kim, D. (2023). Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein. In: Kim, K.K., Subramani, V.K. (eds) Z-DNA. Methods in Molecular Biology, vol 2651. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3084-6_4
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DOI: https://doi.org/10.1007/978-1-0716-3084-6_4
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