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Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniae Under High-Resolution Microscopy

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Bacterial and Archaeal Motility

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2646))

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Abstract

Many phylum Bacteroidetes bacteria are motile without either flagella or pili. These cells move on surfaces such as glass or agar, and a motor generates a propulsion force for the cells via a proton motive force across the cytoplasmic membrane. The gliding motility depends on the helical track of cell adhesin along the longer axis of the cell body. Here, we describe live-cell imaging of gliding motility under optical microscopy, as well as an immunofluorescent labeling method for visualizing helical trajectories.

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Acknowledgments

This work was supported by KAKENHI (16H06230, 20H05543, 21K07020, 22H05066 to DN, and 17K17085, 19K10083 to SS) and funds from the Noguchi Institute to DN. We thank Mark McBride for kindly gifting the antiserum against SprB.

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Authors and Affiliations

  1. Department of Engineering Science, Graduate School of Informatics and Engineering, University of Electro-Communications, Tokyo, Japan

    Daisuke Nakane

  2. Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Tottori, Japan

    Satoshi Shibata

Authors
  1. Daisuke Nakane
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  2. Satoshi Shibata
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Corresponding author

Correspondence to Daisuke Nakane .

Editor information

Editors and Affiliations

  1. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan

    Tohru Minamino

  2. Graduate School of Science, Osaka Metropolitan University, Osaka, Japan

    Makoto Miyata

  3. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan

    Keiichi Namba

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Nakane, D., Shibata, S. (2023). Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniae Under High-Resolution Microscopy. In: Minamino, T., Miyata, M., Namba, K. (eds) Bacterial and Archaeal Motility. Methods in Molecular Biology, vol 2646. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3060-0_22

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  • DOI: https://doi.org/10.1007/978-1-0716-3060-0_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3059-4

  • Online ISBN: 978-1-0716-3060-0

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