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Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2645))

Abstract

Various types of cancer cells enrich or condition the medium that they are cultured in by secreting or shedding proteins and small molecules. These secreted or shed factors are involved in key biological processes, including cellular communication, proliferation, and migration, and are represented by protein families, including cytokines, growth factors, and enzymes. The rapid development of high-resolution mass spectrometry and shotgun strategies for proteome analysis facilitates the identification of these factors in biological models and elucidation of their potential roles in pathophysiology. Hence, the following protocol provides details on how to prepare proteins present in conditioned media for mass spectrometry analysis.

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Author information

Authors and Affiliations

  1. Department of Biology, Maynooth University, National University of Ireland, Kildare, Ireland

    Katie Dunphy & Paul Dowling

  2. Department of Haematology, Mater Misericordiae University Hospital, Dublin, Ireland

    Despina Bazou

Authors
  1. Katie Dunphy
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  2. Despina Bazou
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  3. Paul Dowling
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Corresponding author

Correspondence to Paul Dowling .

Editor information

Editors and Affiliations

  1. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Dania Movia

  2. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Adriele Prina-Mello

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Dunphy, K., Bazou, D., Dowling, P. (2023). Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers. In: Movia, D., Prina-Mello, A. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 2645. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3056-3_18

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  • DOI: https://doi.org/10.1007/978-1-0716-3056-3_18

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3055-6

  • Online ISBN: 978-1-0716-3056-3

  • eBook Packages: Springer Protocols

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