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High-Performance Chromatographic Separation of Cerebrosides

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2625))

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Abstract

High-performance thin layer chromatography (HPTLC) is a very robust, fast, and inexpensive technique that enables separation of complex mixtures. Here, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analysis for structural determination.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL, USA

    Renaud Sicard

  2. VIVEX Biologics, Inc., Miami, United States

    Renaud Sicard

Authors
  1. Renaud Sicard
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Correspondence to Renaud Sicard .

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Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA

    Sanjoy K. Bhattacharya

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sicard, R. (2023). High-Performance Chromatographic Separation of Cerebrosides. In: Bhattacharya, S.K. (eds) Lipidomics. Methods in Molecular Biology, vol 2625. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2966-6_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2966-6_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2965-9

  • Online ISBN: 978-1-0716-2966-6

  • eBook Packages: Springer Protocols

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