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The Post-insertion Method for the Preparation of PEGylated Liposomes

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Liposomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2622))

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Abstract

PEGylation is a crucial process for decorating the surface of liposomes with polyethylene glycol (PEG) for clinical use. This process endows the liposomes extended circulation time and improved stability in vivo. The post-insertion method is one of the well-established techniques for PEGylation. This method requires only one-step incubation to accomplish the transfer of PEGylated lipids from PEGylated lipid-based micelles into the membranes of preformed liposomes.

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References

  1. Torchilin VP (2005) Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discov 4(2):145–160. https://doi.org/10.1038/nrd1632

    Article  CAS  Google Scholar 

  2. Ishida T, Maeda R, Ichihara M et al (2003) Accelerated clearance of PEGylated liposomes in rats after repeated injections. J Control Release 88(1):35–42. https://doi.org/10.1016/S0168-3659(02)00462-5

    Article  CAS  Google Scholar 

  3. Woodle MC, Lasic DD (1992) Sterically stabilized liposomes. Biochim Biophys Acta Rev Biomembr 1113(2):171–199. https://doi.org/10.1016/0304-4157(92)90038-C

    Article  CAS  Google Scholar 

  4. Allen TM (1994) The use of glycolipids and hydrophilic polymers in avoiding rapid uptake of liposomes by the mononuclear phagocyte system. Adv Drug Deliv Rev 13(3):285–309. https://doi.org/10.1016/0169-409X(94)90016-7

    Article  CAS  Google Scholar 

  5. Maeda H (2021) The 35th anniversary of the discovery of EPR effect: a new wave of Nanomedicines for tumor-targeted drug delivery – personal remarks and future prospects. J Pers Med 11:3. https://doi.org/10.3390/jpm11030229

    Article  Google Scholar 

  6. Duggan ST, Keating GM (2011) Pegylated liposomal doxorubicin. Drugs 71(18):2531–2558. https://doi.org/10.2165/11207510-000000000-00000

    Article  CAS  Google Scholar 

  7. Burade V, Bhowmick S, Maiti K et al (2017) Lipodox® (generic doxorubicin hydrochloride liposome injection): in vivo efficacy and bioequivalence versus Caelyx® (doxorubicin hydrochloride liposome injection) in human mammary carcinoma (MX-1) xenograft and syngeneic fibrosarcoma (WEHI 164) mouse models. BMC Cancer 17(1):405. https://doi.org/10.1186/s12885-017-3377-3

    Article  CAS  Google Scholar 

  8. Frampton JE (2020) Liposomal Irinotecan: a review in metastatic pancreatic adenocarcinoma. Drugs 80(10):1007–1018. https://doi.org/10.1007/s40265-020-01336-6

    Article  CAS  Google Scholar 

  9. Zalipsky S, Hansen CB, Lopes de Menezes DE et al (1996) Long-circulating, polyethylene glycol-grafted immunoliposomes. J Control Release 39(2):153–161. https://doi.org/10.1016/0168-3659(95)00149-2

    Article  CAS  Google Scholar 

  10. Moreira JN, Ishida T, Gaspar R et al (2002) Use of the post-insertion technique to insert peptide ligands into pre-formed stealth liposomes with retention of binding activity and cytotoxicity. Pharm Res 19(3):265–269. https://doi.org/10.1023/A:1014434732752

    Article  CAS  Google Scholar 

  11. Zalipsky S, Mullah N, Harding JA et al (1997) Poly(ethylene glycol)-grafted liposomes with oligopeptide or oligosaccharide ligands appended to the termini of the polymer chains. Bioconjug Chem 8(2):111–118. https://doi.org/10.1021/bc9600832

    Article  CAS  Google Scholar 

  12. Ishida T, Iden DL, Allen TM (1999) A combinatorial approach to producing sterically stabilized (stealth) immunoliposomal drugs. FEBS Lett 460(1):129–133. https://doi.org/10.1016/S0014-5793(99)01320-4

    Article  CAS  Google Scholar 

  13. Mare R, Paolino D, Celia C et al (2018) Post-insertion parameters of PEG-derivatives in phosphocholine-liposomes. Int J Pharm 552(1–2):414–421. https://doi.org/10.1016/j.ijpharm.2018.10.028

    Article  CAS  Google Scholar 

  14. Allen TM, Brandeis E, Hansen CB et al (1995) A new strategy for attachment of antibodies to sterically stabilized liposomes resulting in efficient targeting to cancer cells. Biochim Biophys Acta Biomembr 1237(2):99–108. https://doi.org/10.1016/0005-2736(95)00085-H

    Article  Google Scholar 

  15. Bartlett GR (1959) Colorimetric assay methods for free and phosphorylated glyceric acids. J Biol Chem 234(3):469–471

    Article  CAS  Google Scholar 

  16. Silvius JR, Zuckermann MJ (1993) Interbilayer transfer of phospholipid-anchored macromolecules via monomer diffusion. Biochemist 32(12):3153–3161. https://doi.org/10.1021/bi00063a030

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, Tokushima, Japan

    Sherif E. Emam, Nehal E. Elsadek, Taro Shimizu & Tatsuhiro Ishida

  2. Faculty of Pharmacy, Department of Pharmaceutics and Industrial Pharmacy, Zagazig University, Zagazig, Egypt

    Sherif E. Emam

Authors
  1. Sherif E. Emam
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  2. Nehal E. Elsadek
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  3. Taro Shimizu
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  4. Tatsuhiro Ishida
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Corresponding author

Correspondence to Tatsuhiro Ishida .

Editor information

Editors and Affiliations

  1. School of Pharmacy, MCPHS University, Boston, MA, USA

    Gerard G.M. D'Souza

  2. School of Pharmacy, MCPHS University, Boston, MA, USA

    Hongwei Zhang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Emam, S.E., Elsadek, N.E., Shimizu, T., Ishida, T. (2023). The Post-insertion Method for the Preparation of PEGylated Liposomes. In: D'Souza, G.G., Zhang, H. (eds) Liposomes. Methods in Molecular Biology, vol 2622. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2954-3_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2954-3_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2953-6

  • Online ISBN: 978-1-0716-2954-3

  • eBook Packages: Springer Protocols

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