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Isolation and Compositional Analysis of Glycosaminoglycans

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Chemokine-Glycosaminoglycan Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2597))

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Abstract

The compositional and structural analysis of GAGs is challenging due to their heterogenous structures. Strong anion exchange (SAX) HPLC can aid in the compositional analysis of GAGs and can separate complex mixtures based on charge and degree of sulfation. Herein we describe the digestion and release of GAGs from tissue, and the compositional analysis using SAX-HPLC.

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Acknowledgments

This work was supported by NIH grant R24GM137782 to P. A.

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Authors and Affiliations

  1. University of Georgia, Complex Carbohydrate Research Center, Athens, GA, USA

    Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss & Parastoo Azadi

Authors
  1. Stephanie Archer-Hartmann
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  2. Lauren E. Pepi
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  3. Christian Heiss
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  4. Parastoo Azadi
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Corresponding author

Correspondence to Parastoo Azadi .

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Editors and Affiliations

  1. Biodesign Institute, Arizona State University, Tempe, AZ, USA

    Alexandra R. Lucas

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Archer-Hartmann, S., Pepi, L.E., Heiss, C., Azadi, P. (2023). Isolation and Compositional Analysis of Glycosaminoglycans. In: Lucas, A.R. (eds) Chemokine-Glycosaminoglycan Interactions. Methods in Molecular Biology, vol 2597. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2835-5_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2835-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2834-8

  • Online ISBN: 978-1-0716-2835-5

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