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Exosomal MicroRNAs: Comprehensive Methods from Exosome Isolation to miRNA Extraction and Purity Analysis

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MicroRNA Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2595))

Abstract

Exosomes are extracellular vesicles secreted by cells with a key role in a wide range of biological processes including cancer. These vesicles are involved in intercellular communication and deliver diverse cargo molecules, including miRNAs (exo-miRNAs), to recipient cells affecting their physiology. Exo-miRNAs have a role in promoting tumor, progression, metastatization, and remodeling of tumor microenvironment, therefore making them interesting biomarkers to study.

Here we provide a detailed technical protocol for exosome isolation (which can be applied to cell culture as well as physiological fluids), validation of their vesicular identity, miRNA extraction, and quantitative and qualitative analysis to evaluate the sample purity and concentration.

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Change history

  • 29 January 2023

    In the original version of this book, the first and the last names of the authors of Chapter 5 were in a flipped format (D’Agostino Erika, Muro Annamaria, Sgueglia Giulia, Massaro Crescenzo, Dell’Aversana Carmela, and Altucci Lucia). This has been rectified in the updated version of this book.

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Author information

Author notes

  1. Authors ‘Dell’Aversana Carmela’ and ‘Altucci Lucia’ have been contributed equally to this work

Authors and Affiliations

  1. Department of Precision Medicine, Università degli studi della Campania “Luigi Vanvitelli”, Naples, Italy

    Erika D’Agostino, Annamaria Muro, Giulia Sgueglia, Crescenzo Massaro, Carmela Dell’Aversana & Lucia Altucci

  2. Università degli studi di Napoli “Federico II”, Naples, Italy

    Annamaria Muro

  3. Institute Experimental Endocrinology and Oncology ‘Gaetano Salvatore’ (IEOS)-National Research Council (CNR), Naples, Italy

    Carmela Dell’Aversana

  4. BIOGEM, Ariano Irpino, Italy

    Lucia Altucci

Authors
  1. Erika D’Agostino
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  2. Annamaria Muro
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  3. Giulia Sgueglia
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  4. Crescenzo Massaro
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  5. Carmela Dell’Aversana
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  6. Lucia Altucci
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Corresponding authors

Correspondence to Carmela Dell’Aversana or Lucia Altucci .

Editor information

Editors and Affiliations

  1. Department of Science, South East Technological University, Waterford, Ireland

    Sweta Rani

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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D’Agostino, E., Muro, A., Sgueglia, G., Massaro, C., Dell’Aversana, C., Altucci, L. (2023). Exosomal MicroRNAs: Comprehensive Methods from Exosome Isolation to miRNA Extraction and Purity Analysis. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 2595. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2823-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2823-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2822-5

  • Online ISBN: 978-1-0716-2823-2

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