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Synthesis of siRNA-Conjugated Dextran-Coated Iron Oxide Nanoparticles for Islet Protection During Transplantation and Noninvasive Imaging

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Type-1 Diabetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2592))

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Abstract

Pancreatic islet transplantation (Tx) has a lifesaving potential for type 1 diabetes (T1D) patients. Islet damage during and after transplantation is one of the major reasons hampering its wide clinical application. Inability to monitor transplanted islets also severely limits our understanding of mechanisms regarding declining graft function after transplantation. Our team has proposed to use magnetic nanoparticles conjugated to siRNA (MN-siRNA) to label islets prior to transplantation with two goals in mind: to protect them from damage by silencing harmful genes and to monitor them after transplantation using noninvasive magnetic resonance imaging (MRI). This manuscript provides a step-by-step protocol for the synthesis and characterization of MN-siRNA probes.

This work was supported in part by R01DK105503, R01DK105468, U19AI131474 and P01AI045897. All authors report no conflict of interest in regard to the current publication.

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Author information

Authors and Affiliations

  1. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Neil Robertson, Ping Wang, Nazanin Talebloo & Anna Moore

  2. Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA

    Neil Robertson, Ping Wang & Anna Moore

  3. Department of Chemistry, College of Natural Sciences, Michigan State University, East Lansing, MI, USA

    Nazanin Talebloo

  4. Columbia Center for Translational Immunology, Department of Surgery, Columbia University Irving Medical Center, New York, NY, USA

    Kazuhiko Yamada

  5. Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA

    Kazuhiko Yamada

Authors
  1. Neil Robertson
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  2. Ping Wang
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  3. Nazanin Talebloo
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  4. Kazuhiko Yamada
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  5. Anna Moore
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Corresponding author

Correspondence to Anna Moore .

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Editors and Affiliations

  1. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Anna Moore

  2. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Ping Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Robertson, N., Wang, P., Talebloo, N., Yamada, K., Moore, A. (2023). Synthesis of siRNA-Conjugated Dextran-Coated Iron Oxide Nanoparticles for Islet Protection During Transplantation and Noninvasive Imaging. In: Moore, A., Wang, P. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 2592. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2807-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2807-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2806-5

  • Online ISBN: 978-1-0716-2807-2

  • eBook Packages: Springer Protocols

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