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Detection of Autophagy in Plants by Fluorescence Microscopy

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Plant Proteostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2581))

Abstract

Autophagy is a key process for degradation and recycling of proteins or organelles in eukaryotes. Autophagy in plants has been shown to function in stress responses, pathogen immunity, and senescence, while a basal level of autophagy plays a housekeeping role in cells. Upon activation of autophagy, vesicles termed autophagosomes are formed to deliver proteins or organelles to the vacuole for degradation. The number of autophagosomes can thus be used to indicate the level of autophagy. Here we describe two common methods used for detection of autophagosomes, staining of autophagosomes with the fluorescent dye monodansylcadaverine and expression of a fusion between GFP and the autophagosomal membrane protein ATG8.

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Acknowledgments

This work was supported by grant no. IOS-1353867 from the National Science Foundation to D.C.B. We thank Xiaochen Yang for providing GFP images for Fig. 1.

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Authors and Affiliations

  1. Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA

    Yunting Pu & Diane C. Bassham

  2. Plant Sciences Institute, Iowa State University, Ames, IA, USA

    Diane C. Bassham

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  1. Yunting Pu
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  2. Diane C. Bassham
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Correspondence to Diane C. Bassham .

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Editors and Affiliations

  1. Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Barcelona, Spain

    L. Maria Lois

  2. Faculty of Biology, University of Freiburg, Freiburg, Germany

    Marco Trujillo

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Pu, Y., Bassham, D.C. (2023). Detection of Autophagy in Plants by Fluorescence Microscopy. In: Lois, L.M., Trujillo, M. (eds) Plant Proteostasis. Methods in Molecular Biology, vol 2581. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2784-6_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2784-6_11

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