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Targeted Manipulation of Histone Modification in Medaka Embryos

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Epigenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2577))

Abstract

Recent development of targeted manipulation of histone modification enables us to experimentally and directly test the functional relevance of histone modifications accumulated at specific genomic regions. In particular, dCas9 epigenome editing has been widely used for site-specific manipulation of epigenetic modification. Here, we describe how to apply dCas9 epigenome editing in fish (medaka, Oryzias latipes) embryos and how to analyze induced changes in histone modification.

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Acknowledgments

This work was supported by JSPS Grant-in-Aid for JSPS Research Fellow Grant Number JP18J21761 to H.S.F., by Japan Agency for Medical Research and Development (AMED) under Grant Number JP18gm1110007h0001 to H.T., by Japan Society for the Promotion of Science (JSPS) grant number JP21K06013, and by Grant-in-Aid for Scientific Research on Innovative Areas grant number JP21H00245 to R.N.

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Authors and Affiliations

  1. Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan

    Hiroto S. Fukushima, Hiroyuki Takeda & Ryohei Nakamura

Authors
  1. Hiroto S. Fukushima
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  2. Hiroyuki Takeda
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  3. Ryohei Nakamura
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Corresponding author

Correspondence to Ryohei Nakamura .

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Editors and Affiliations

  1. Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

  2. Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Takuro Horii

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fukushima, H.S., Takeda, H., Nakamura, R. (2023). Targeted Manipulation of Histone Modification in Medaka Embryos. In: Hatada, I., Horii, T. (eds) Epigenomics. Methods in Molecular Biology, vol 2577. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2724-2_20

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  • DOI: https://doi.org/10.1007/978-1-0716-2724-2_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2723-5

  • Online ISBN: 978-1-0716-2724-2

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